https://eln.excellgen.com/api.php?action=feedcontributions&feedformat=atom&user=Xiaojing.Wang
ELN - User contributions [en]
2026-10-18T06:13:33Z
User contributions
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=File:1283_0810_Digestion.JPG&diff=5524
File:1283 0810 Digestion.JPG
2012-08-10T18:10:19Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1283_0809_Amp.JPG&diff=5512
File:1283 0809 Amp.JPG
2012-08-09T20:01:11Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1291_pET30b-TAT-NLS-sso7d&diff=5508
1291 pET30b-TAT-NLS-sso7d
2012-08-09T18:49:59Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1291 pET30b-TAT-NLS-sso7d''' ==<br />
<br />
'''Purpose:''' Cloning of 1291 pET30b-TAT-NLS-sso7d <br />
<br />
'''Date: '''July 31, 2012<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1291 <br />
<br />
'''Experimenters: '''Jennifer Truong <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning<br> ===<br />
<br />
Date: July 31, 2012<br> <br />
<br />
[[Image:1291 Amp.JPG]]<br> <br />
<br />
Gene synthesis of PCR&nbsp;product. Expected size ~100 bp<br> <br />
<br />
See lane 2<br> <br />
<br />
Cut out and purify band, digest with RE&nbsp; Nde1 + Kpn1&nbsp; (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)<br> <br />
<br />
[[Image:1291 Digestion.JPG]] <br />
<br />
Cut out bands, purify and ligate over night <br />
<br />
Date:&nbsp;August 6, 2012<br><br />
<br />
MiniPrep screening came back all negative, started all over again<br><br />
<br />
[[Image:1291_0806_Amp.JPG]]<br><br />
<br />
Gene synthesis of PCR product. Expected size ~100 bp<br />
<br />
See lane 2<br />
<br />
Cut out and purify band, digest with RE Nde1 + Kpn1 (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)<br><br />
<br />
[[Image:1291_0806_Digestion.JPG]]<br><br />
<br />
Cut out bands, purify and ligate over night <br><br />
<br />
=== <u></u>Colony Screening<br> ===<br />
<br />
[[Image:1291 Screening.JPG]]<br> <br />
<br />
Digested MiniPrep with RE Nde1 + Kpn1, no inserts<br> <br />
<br />
Date:&nbsp;August 9, 2012<br> <br />
<br />
[[Image:1291_0809_Screening.JPG]]<br />
<br />
Digested MIniPrep with RE&nbsp;Nde1 and Kpn1, faint inserts at expected ~100 bp<br />
<br />
1, 2, 3, 5 and 6 may be correct<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1271_0808_Amp.JPG&diff=5506
File:1271 0808 Amp.JPG
2012-08-09T18:47:59Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1271_0809_Vector.JPG&diff=5505
File:1271 0809 Vector.JPG
2012-08-09T18:42:46Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_0809_Screening.JPG&diff=5504
File:1291 0809 Screening.JPG
2012-08-09T18:42:37Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1271_0809_Amp.JPG&diff=5502
File:1271 0809 Amp.JPG
2012-08-09T14:47:22Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1292_0807_Screening_2.JPG&diff=5490
File:1292 0807 Screening 2.JPG
2012-08-07T19:01:01Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1292_0807_Screening.JPG&diff=5487
File:1292 0807 Screening.JPG
2012-08-07T16:45:47Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1290_0807_Screening.JPG&diff=5486
File:1290 0807 Screening.JPG
2012-08-07T16:45:29Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1283_0807_Amp.JPG&diff=5480
File:1283 0807 Amp.JPG
2012-08-07T14:46:31Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1275_pTXB1-RNaseT&diff=5473
1275 pTXB1-RNaseT
2012-08-06T19:55:40Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1275 pTXB1-RNaseT''' ==<br />
<br />
'''Purpose:''' Cloning of 1275 pTXB1-RNaseT<br />
<br />
'''Date: '''July 30, 2012<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1275 <br />
<br />
'''Experimenters: '''Jennifer Truong <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
Date:&nbsp;July 30, 2012<br> <br />
<br />
[[Image:0730 Amp.JPG]]<br> <br />
<br />
See lane 3<br> <br />
<br />
<br> <br />
<br />
PCR using genomic DNA from K-12 substr. MG1655 <br />
<br />
<br> <br />
<br />
RnaseT-Nde1-F AGCTCTCATatgtccgataacgctcaacttac<br>RnaseT-Xho1-R gtctcgctcgagcacctcttcggcggcagatag<br>clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK<br> <br />
<br />
Expected size 666 bp<br> <br />
<br />
Cut out band and save in box<br />
<br />
Date: August 1, 2012<br><br />
<br />
[[Image:0801_Digestion.JPG]]<br><br />
<br />
Digested insert (lane 3)&nbsp;with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 3, purify and ligate together <br><br />
<br />
=== Colony Screening ===<br />
<br />
Date:&nbsp;August 6, 2012 <br />
<br />
Digested MiniPrep with RE&nbsp;Xho1 and Nde1, expected size 666 bp <br />
<br />
[[Image:1275 0806 Screening.JPG]] <br />
<br />
Samples 1, 3, 4 5(?)&nbsp;may be correct <br />
<br />
Send sample 3 to sequencing<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1274_pTXB1-RnaseH2&diff=5471
1274 pTXB1-RnaseH2
2012-08-06T19:51:33Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1274 pTXB1-RnaseH2''' ==<br />
<br />
'''Purpose:''' Cloning of 1274 pTXB1-RnaseH2 <br />
<br />
'''Date: '''July 30, 2012<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1274 <br />
<br />
'''Experimenters: '''Jennifer Truong <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
Date:&nbsp;July 30, 2012<br> <br />
<br />
[[Image:0730 Amp.JPG]]<br> <br />
<br />
See lane 2<br> <br />
<br />
<br> <br />
<br />
PCR using genomic DNA from Escherichia coli str. K-12 substr. MG1655<br>RnaseH2-Nde1-F AGCTCTCATatgatcgaatttgtttatccgcac<br>RnaseH2-Xho1-R gtctcgctcgagggacgcaagtcccagtgcgcgt<br>clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK <br />
<br />
Expected size 615 bp<br> <br />
<br />
Cut out band and save in box <br />
<br />
Date: August 1, 2012<br> <br />
<br />
[[Image:0801 Digestion.JPG]]<br> <br />
<br />
Digested insert (lane 2)&nbsp;with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 2, purify and ligate together <br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date:&nbsp;August 6, 2012<br />
<br />
Digested MiniPrep with RE Nde1 and Xho1, expected size 615 bp<br />
<br />
[[Image:1274_0806_Screening.JPG]]<br />
<br />
Bad digestion gel, digest again tomorrow<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1273_pTXB1-RnaseH&diff=5470
1273 pTXB1-RnaseH
2012-08-06T19:50:26Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1273 pTXB1-RnaseH''' ==<br />
<br />
'''Purpose:''' Cloning of 1273 pTXB1-RnaseH <br />
<br />
'''Date: '''July 30, 2012<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1273 <br />
<br />
'''Experimenters: '''Jennifer Truong <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
Date:&nbsp;July 30, 2012<br> <br />
<br />
[[Image:0730 Amp.JPG]]<br> <br />
<br />
See lane 1<br> <br />
<br />
PCR using genomic DNA from K-12 substr. MG1655<br>RnaseH-Nde1-F AGCTCTCATatgcttaaacaggtagaaattttc<br>RnaseH-Xho1-R gtctcgctcgagaacttcaacttggtagcctg <br />
<br />
Clone into Nde1 + Xho1 digested 1254 pTXB1-PT-ELK<br> <br />
<br />
Expected size 496 bp<br> <br />
<br />
Cut out band and save in box <br />
<br />
Date: August 1, 2012<br> <br />
<br />
[[Image:0801 Digestion.JPG]]<br> <br />
<br />
Digested insert (lane 1)&nbsp;with RE Nde1 + Xho1, vector is not good, digest again tomorrow, join with lane 1, purify and ligate together <br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date:&nbsp;August 6, 2012<br> <br />
<br />
Digested MiniPrep with RE Nde1 + Xho1, expected size 496 bp<br> <br />
<br />
[[Image:1273 0806 Screening.JPG]]<br> <br />
<br />
Sample 1-5 may be correct <br />
<br />
Send sample 3 to sequencing<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1134_pTXB1-Ecoli-Ligase&diff=5467
1134 pTXB1-Ecoli-Ligase
2012-08-06T19:44:54Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1134 pTXB1-Ecoli-Ligase''' ==<br />
<br />
'''Purpose:''' Cloning of 1134 pTXB1-Ecoli-Ligase<br />
<br />
'''Date: '''July 23, 2012<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1134 <br />
<br />
'''Experimenters: '''Jennifer Truong <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
Date:&nbsp;July 24, 2012<br> <br />
<br />
[[Image:0724 Amp.JPG]]<br> <br />
<br />
See lane 3, expected size 2017 bp<br> <br />
<br />
PCR using these primers and genomic DNA fromm MG 1665 <br>Ecoli_lig-Nde1-F<br>atcatcCATatggaatcaatcgaacaacaac <br />
<br />
Ecoli_lig-Xho1-R <br />
<br />
(gaaatgctgcgtttgctgggtagc CTCGAG catgca)<br>tgcatgCTCGAGgctacccagcaaacgcagcatttc <br />
<br />
Clone into pTXB1-PT-ELK (1254)&nbsp;with RE&nbsp;(Nde1 + Xho1).<br> <br />
<br />
[[Image:0726 Digestion.JPG]] <br />
<br />
See lane 8, digested insert and vector together in the same lane. Insert is missing, need to reamplify. Save vector <br />
<br />
[[Image:0724 Amp 2.JPG]]<br> <br />
<br />
See lane 2, expected size 2017 bp <br />
<br />
PCR using these primers and genomic DNA from MG 1665<br>Ecoli_lig-Nde1-F<br>atcatcCATatggaatcaatcgaacaacaac <br />
<br />
Ecoli_lig-Xho1-R <br />
<br />
(gaaatgctgcgtttgctgggtagc CTCGAG catgca)<br>tgcatgCTCGAGgctacccagcaaacgcagcatttc <br />
<br />
Clone into pTXB1-PT-ELK (1254) with RE (Nde1 + Xho1).<br> <br />
<br />
[[Image:0724 Digestion.JPG]]<br> <br />
<br />
Digested with Nde1 and Xho1 (see lane 2), cut out band, purify and ligate together with vector saved form earlier. Ligate over night, transform tomorrow morning<br> <br />
<br />
<br> <br />
<br />
Date:&nbsp;August 2, 2012<br> <br />
<br />
Screening came back bad, started all over again<br> <br />
<br />
[[Image:0802 Amp.JPG]]<br> <br />
<br />
See lane 3, expected size 2017 bp <br />
<br />
PCR using these primers and genomic DNA fromm MG 1665<br>Ecoli_lig-Nde1-F<br>atcatcCATatggaatcaatcgaacaacaac <br />
<br />
Ecoli_lig-Xho1-R <br />
<br />
(gaaatgctgcgtttgctgggtagc CTCGAG catgca)<br>tgcatgCTCGAGgctacccagcaaacgcagcatttc <br />
<br />
Clone into pTXB1-PT-ELK (1254) with RE (Nde1 + Xho1).<br> <br />
<br />
[[Image:1134_Digestion.JPG]]<br />
<br />
Ligate together, transform<br />
<br />
=== <u></u>Colony Screening<br> ===<br />
<br />
Date:&nbsp;July 27, 2012 <br />
<br />
[[Image:0727 Digestion.JPG]] <br />
<br />
Digested MiniPrep with RE Nde1 and Xho1, expected size 2017 bp <br />
<br />
See lanes 19- 24 <br />
<br />
Lane 21 (sample 3)&nbsp;may have insert?&nbsp;Send sample 3 to sequencing <br />
<br />
Date:&nbsp;August 6, 2012<br><br />
<br />
[[Image:1134_0806_Screening.JPG]]<br><br />
<br />
Digested MiniPrep with RE Nde1 and Xho1, expected size 2017 bp <br><br />
<br />
No inserts<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
<br> <br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1134_0806_Screening.JPG&diff=5466
File:1134 0806 Screening.JPG
2012-08-06T19:44:12Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1117_0806_Screening.JPG&diff=5462
File:1117 0806 Screening.JPG
2012-08-06T19:30:55Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1118_0806_Screening.JPG&diff=5461
File:1118 0806 Screening.JPG
2012-08-06T19:30:50Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1141_0806_Screening.JPG&diff=5460
File:1141 0806 Screening.JPG
2012-08-06T19:30:39Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1273_0806_Screening.JPG&diff=5459
File:1273 0806 Screening.JPG
2012-08-06T19:30:33Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1274_0806_Screening.JPG&diff=5458
File:1274 0806 Screening.JPG
2012-08-06T19:30:14Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1275_0806_Screening.JPG&diff=5457
File:1275 0806 Screening.JPG
2012-08-06T19:30:08Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1277_0806_Screening.JPG&diff=5456
File:1277 0806 Screening.JPG
2012-08-06T19:30:01Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_0806_Digestion.JPG&diff=5455
File:1291 0806 Digestion.JPG
2012-08-06T19:29:56Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_0806_Amp.JPG&diff=5454
File:1291 0806 Amp.JPG
2012-08-06T19:29:51Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_Screening.JPG&diff=5438
File:1291 Screening.JPG
2012-08-03T17:18:25Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1283_Screening.JPG&diff=5435
File:1283 Screening.JPG
2012-08-03T17:14:46Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1290_0802_Digestion.JPG&diff=5420
File:1290 0802 Digestion.JPG
2012-08-02T19:13:12Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1292_Vector.JPG&diff=5419
File:1292 Vector.JPG
2012-08-02T19:13:02Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1117_Digestion.JPG&diff=5413
File:1117 Digestion.JPG
2012-08-02T17:00:59Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1118_Digestion.JPG&diff=5412
File:1118 Digestion.JPG
2012-08-02T17:00:54Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1134_Digestion.JPG&diff=5411
File:1134 Digestion.JPG
2012-08-02T17:00:49Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1141_Digestion.JPG&diff=5410
File:1141 Digestion.JPG
2012-08-02T17:00:44Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1290_0802_Amp.JPG&diff=5409
File:1290 0802 Amp.JPG
2012-08-02T17:00:39Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:0801_Digestion.JPG&diff=5400
File:0801 Digestion.JPG
2012-08-02T13:50:08Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:0802_Amp.JPG&diff=5399
File:0802 Amp.JPG
2012-08-02T13:49:57Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1290_Amp.JPG&diff=5395
File:1290 Amp.JPG
2012-08-01T18:38:31Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1292_Amp.JPG&diff=5394
File:1292 Amp.JPG
2012-08-01T18:38:26Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1290_Digestion.JPG&diff=5393
File:1290 Digestion.JPG
2012-08-01T18:38:22Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1292_Digestion_Insert.JPG&diff=5392
File:1292 Digestion Insert.JPG
2012-08-01T18:38:16Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_Digestion.JPG&diff=5386
File:1291 Digestion.JPG
2012-07-31T19:45:31Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1291_Amp.JPG&diff=5374
File:1291 Amp.JPG
2012-07-31T18:07:45Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1283_Digestion.JPG&diff=5372
File:1283 Digestion.JPG
2012-07-31T17:06:46Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:0731_Amp.JPG&diff=5370
File:0731 Amp.JPG
2012-07-31T14:37:32Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1106_Digestion.JPG&diff=5357
File:1106 Digestion.JPG
2012-07-30T20:25:19Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1270_pTXB1-RNaseT1&diff=5356
1270 pTXB1-RNaseT1
2012-07-30T18:36:41Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1270 pTXB1-RNaseT1''' ==<br />
<br />
'''Purpose:''' Cloning of 1270 pTXB1-RNaseT1 by gene synthesis <br> <br />
<br />
'''Date: '''July 26, 2012<br> <br />
<br />
'''Notebook:''' 0 <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1270 <br />
<br />
'''Experimenters:''' Jennifer Truong<br> <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning<br> ===<br />
<br />
Date:&nbsp;July 26, 2012<br> <br />
<br />
[[Image:0726 Amp.JPG]] <br />
<br />
Amplified DNA sequence by gene synthesis, expected size ~400 bp (see lane 2) <br />
<br />
Cut out band and purified <br />
<br />
Digested with vector 1254 with RE Xho1 and Nde1 <br />
<br />
[[Image:1270 digestion.JPG]] <br />
<br />
See lanes 3 (insert)&nbsp;and 4 (vector) Cut out, purify and ligate together. <br />
<br />
Transform <br />
<br />
=== Colony Screening ===<br />
<br />
Date:&nbsp;July 30, 2012<br />
<br />
[[Image:1268_1270_Screening.JPG]]<br />
<br />
Digested MiniPrep with Nde1 and Xho1 RE<br>Expected size 400 bp<br />
<br />
Samples 4 (lane 8), 4 (lane 10)&nbsp;and 6 (lane 12) may have positive inserts <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion'''<br> ==<br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=1268_pTXB1-RNaseA&diff=5354
1268 pTXB1-RNaseA
2012-07-30T18:25:54Z
<p>Xiaojing.Wang: </p>
<hr />
<div>== '''Cloning of 1268 pTXB1-RNaseA''' ==<br />
<br />
'''Purpose:''' Cloning of 1268 pTXB1-RNaseA by gene synthesis <br> <br />
<br />
'''Date: '''July 26, 2012<br> <br />
<br />
'''Notebook:''' 0 <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: '''1268 <br />
<br />
'''Experimenters:''' Jennifer Truong<br> <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning<br> ===<br />
<br />
Date:&nbsp;July 26, 2012<br> <br />
<br />
[[Image:0726 Amp.JPG]] <br />
<br />
See lane 1, amplified DNA sequence by gene synthesis (25 cycles, HotStartPhusion), expected size ~400 bp <br />
<br />
Cut out band, purify and digest with 1254 vector with RE Nde1 and Xho1 <br />
<br />
[[Image:1268_digestion.JPG]]<br />
<br />
See lane 1 (insert)&nbsp;and lane 2 (vector), cut out purify and ligate together. Transform<br />
<br />
=== Colony Screening ===<br />
<br />
Date: July 30, 2012<br />
<br />
[[Image:1268_1270_Screening.JPG]]<br />
<br />
Digested MiniPrep with RE Nde1 and Xho1<br />
<br />
Expected size ~400 bp<br />
<br />
Samples 3 and 5 (lanes 3 and 5 respectively) may be positive for insert<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion'''<br> ==<br />
<br />
[[Category:Cloning]]</div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1268_1270_Screening.JPG&diff=5351
File:1268 1270 Screening.JPG
2012-07-30T18:21:54Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:0730_Amp.JPG&diff=5342
File:0730 Amp.JPG
2012-07-30T18:13:37Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang
https://eln.excellgen.com/index.php?title=File:1106_Screening.JPG&diff=5340
File:1106 Screening.JPG
2012-07-30T18:10:17Z
<p>Xiaojing.Wang: </p>
<hr />
<div></div>
Xiaojing.Wang