https://eln.excellgen.com/index.php?action=history&feed=atom&title=1004-pET8XHis-PKAalpha 2026-10-18T03:12:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1004-pET8XHis-PKAalpha&diff=14441&oldid=prev 2015-05-16T19:07:52Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1004-pET8XHis-PKAalpha&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1004-pET8XHis-PKAalpha by PCR<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-7--<br /> <br /> &#039;&#039;&#039;Notebook:003732&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID:1004&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-2-7<br /> <br /> [[File:546+1004 PCR 2-8-2012.JPG|RTENOTITLE]]<br /> <br /> &amp;nbsp;PCR&amp;nbsp;: template is pSpeedET-PKAalapha<br /> <br /> PKAalpha-Nco1-F + PKAalpha-BamH1-R<br /> <br /> [[File:323+1004 clone 2-8-2012.JPG|RTENOTITLE]]<br /> <br /> {| style=&quot;width: 334px; height: 64px&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | band 3&lt;br/&gt;<br /> | band 4&lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | PCR product&lt;br/&gt;<br /> | 286pET8XHis&lt;br/&gt;<br /> |-<br /> | digest enzymes&lt;br/&gt;<br /> | colspan=&quot;2&quot; | Nco1+BamH1&lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-2-10<br /> <br /> Make miniprep and digest with&amp;nbsp; Nco1+BamH1<br /> <br /> [[File:323+1004 digest detect 2-10-2012.JPG|RTENOTITLE]]<br /> <br /> clone 1 to 6 may be right, need to sequence<br /> <br /> &lt;br/&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;lt;br&amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:Cloning]]</div>

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