https://eln.excellgen.com/index.php?action=history&feed=atom&title=1005-pBad24-Tat-sfGFP 1005-pBad24-Tat-sfGFP - Revision history 2026-10-18T02:19:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1005-pBad24-Tat-sfGFP&diff=14116&oldid=prev Excellgen at 17:44, 2 April 2015 2015-04-02T17:44:29Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1005-pBad24-Tat-sfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1005-pBad24-Tat-sfGFP by PCR<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-10--2012-2-17<br /> <br /> &#039;&#039;&#039;Notebook:003737&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID:1005&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-2-10<br /> <br /> [[File:1005 PCR 2-10-2012.JPG|RTENOTITLE]]<br /> <br /> Mix the His-Tat-NLS-1 to&amp;nbsp;His-Tat-NLS-5 oligos and oligos for pBad24-sfGFP (546), gene synthesis<br /> <br /> primer F:His-Tat-NLS-1<br /> <br /> primer R:sfGFP20<br /> <br /> [[File:546+1002+1005 clone 2-15-2012.JPG|RTENOTITLE]]<br /> <br /> {| style=&quot;width: 312px; height: 96px&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | band 5&lt;br/&gt;<br /> | band 6&lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | PCR product&lt;br/&gt;<br /> | pBad24 545&lt;br/&gt;<br /> |-<br /> | digest enzymes&lt;br/&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + HindIII&lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-2-17<br /> <br /> Make miniprep and digest with Nco1 + HindIII<br /> <br /> [[File:546+1005 digest detect 2-17-2012.JPG|RTENOTITLE]]<br /> <br /> clone 1,2,3,5,6&amp;nbsp; may be right, need to sequence<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;lt;br&amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt;<br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:Cloning]]</div> Excellgen