https://eln.excellgen.com/index.php?action=history&feed=atom&title=1007-pLVX-loxP-sfGFP 1007-pLVX-loxP-sfGFP - Revision history 2026-10-18T02:17:13Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1007-pLVX-loxP-sfGFP&diff=14050&oldid=prev Excellgen at 19:50, 26 March 2015 2015-03-26T19:50:52Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1007(548)-pLVX-loxP-sfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1007-pLVX-loxP-sfGFP from 535-1 pAAV-CMV-DIO-sfGFP to PLVX-plus2,164<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-7--<br /> <br /> &#039;&#039;&#039;Notebook:003732&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID:1007&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying<br /> <br /> NOTE: change &#039;&#039;&#039;548&#039;&#039;&#039; into &#039;&#039;&#039;1007&#039;&#039;&#039;<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date:2012-2-7<br /> <br /> [[File:548 clone 2-7-2012.JPG|RTENOTITLE]]<br /> <br /> {| style=&quot;width: 343px; height: 112px&quot; border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | band 1&lt;br/&gt;<br /> | band 2&lt;br/&gt;<br /> |-<br /> | &lt;br/&gt;<br /> | 535-1 pAAV-CMV-DIO-sfGFP&lt;br/&gt;<br /> | PLVX-plus2,164&lt;br/&gt;<br /> |-<br /> | digest enzymes&lt;br/&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp; EcoR1 + BamH1&lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {| border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-2-9<br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1 + BamH1<br /> <br /> [[File:534+548 digest detect 2-9-2012.JPG|RTENOTITLE]]<br /> <br /> clone 1 to 8 may be right, need to transfect into cells<br /> <br /> [[File:1007-1 transfection 1-21-2012.JPG|168x156px|1007-1 transfection 1-21-2012.JPG]][[File:1007-2 transfection 1-21-2012.JPG|171x153px|1007-2 transfection 1-21-2012.JPG]][[File:1007-3 transfection 1-21-2012.JPG|171x152px|1007-3 transfection 1-21-2012.JPG]][[File:1007-4 transfection 1-21-2012.JPG|160x152px|1007-4 transfection 1-21-2012.JPG]][[File:1007-5 transfection 1-21-2012.JPG|151x153px|1007-5 transfection 1-21-2012.JPG]]<br /> <br /> <br /> <br /> <br /> <br /> [[File:1007-6 transfection 1-21-2012.JPG|170x151px|1007-6 transfection 1-21-2012.JPG]][[File:1007-7 transfection 1-21-2012.JPG|153x151px|1007-7 transfection 1-21-2012.JPG]][[File:1007-8 transfection 1-21-2012.JPG|146x152px|1007-8 transfection 1-21-2012.JPG]]<br /> <br /> transfection result: all positive<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> {| border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {| cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:Cloning]]</div> Excellgen