https://eln.excellgen.com/index.php?action=history&feed=atom&title=1008-pLVX-loxP-sfGFP-loxP 2026-10-18T05:18:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1008-pLVX-loxP-sfGFP-loxP&diff=3366&oldid=prev 2012-02-21T20:48:24Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1008-pLVX-loxP-sfGFP-loxP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1008-pLVX-loxP-sfGFP-loxP by PCR <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-10--2-15 <br /> <br /> &#039;&#039;&#039;Notebook:003738&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1008&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-2-10 &lt;br&gt; <br /> <br /> [[Image:1008+1009 PCR 2012-2-10.JPG]]&lt;br&gt; <br /> <br /> template:254, pAAV.CMV-MCS&lt;br&gt; <br /> <br /> primer F:hGHpA-EcoR1-F <br /> <br /> primer R:hGHpA-loxP-Xho1-R&lt;br&gt; <br /> <br /> [[Image:1008+1009 clone 2012-2-13.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 274px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1007,pLVX-loxP-sfGFP&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | EcoR1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012- 2-15&lt;br&gt; <br /> <br /> [[Image:1008+1009 digest detect 2-15-2012.JPG]] <br /> <br /> clone 2,3,4,5,6 may be right <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1008-2 transfection 1-21-2012.JPG|167x157px|1008-2 transfection 1-21-2012.JPG]][[Image:1008-3 transfection 1-21-2012.JPG|155x158px|1008-3 transfection 1-21-2012.JPG]][[Image:1008-4 transfection 1-21-2012.JPG|165x157px|1008-4 transfection 1-21-2012.JPG]][[Image:1008-5 transfection 1-21-2012.JPG|163x157px|1008-5 transfection 1-21-2012.JPG]][[Image:1008-6 transfection 1-21-2012.JPG|149x156px|1008-6 transfection 1-21-2012.JPG]] <br /> <br /> transfection result: 2-6 positive <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu