https://eln.excellgen.com/index.php?action=history&feed=atom&title=1009-pLVX-TurboRFP 1009-pLVX-TurboRFP - Revision history 2026-10-18T08:19:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1009-pLVX-TurboRFP&diff=3781&oldid=prev Paul.xu at 20:01, 6 April 2012 2012-04-06T20:01:24Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1009-pLVX-TurboRFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1009-pLVX-TurboRFP by genesynthesis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-10--2-15 <br /> <br /> &#039;&#039;&#039;Notebook:003738&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1009&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-2-10 &lt;br&gt; <br /> <br /> [[Image:1008+1009 PCR 2012-2-10.JPG]]&lt;br&gt; <br /> <br /> template:TurboRFP-1 to TurboRFP-20 mixture&lt;br&gt; <br /> <br /> primer F:TurboRFP-1&lt;br&gt; <br /> <br /> primer R:TurboRFP-20&lt;br&gt; <br /> <br /> [[Image:1008+1009 clone 2012-2-13.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 3&lt;br&gt; <br /> | band 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pLVX-Plus2 164&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xho1 + Xba1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-2-15 <br /> <br /> Make miniprep and digest with Xho1 + Xba1&lt;br&gt; <br /> <br /> [[Image:1008+1009 digest detect 2-15-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1009-pLVX-TurboRFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1009-pLVX-TurboRFP by genesynthesis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-2-16-- <br /> <br /> &#039;&#039;&#039;Notebook:003745--&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1009&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-2-10 &lt;br&gt; <br /> <br /> [[Image:1008+1009 PCR 2012-2-10.JPG]]&lt;br&gt; <br /> <br /> template:TurboRFP-1 to TurboRFP-20 mixture&lt;br&gt; <br /> <br /> primer F:TurboRFP-1&lt;br&gt; <br /> <br /> primer R:TurboRFP-20&lt;br&gt; <br /> <br /> [[Image:1009 clone 2-16-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pLVX-Plus2 164&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xho1 + Xba1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012 <br /> <br /> Make miniprep and digest with Xho1 + Xba1&lt;br&gt; <br /> <br /> [[Image:1009 digest detect 2-21-2012.JPG]] <br /> <br /> no insert <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1009-pLVX-TurboRFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1009-pLVX-TurboRFP by genesynthesis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-3-13--2012-3-15 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1009&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-3-15&lt;br&gt; <br /> <br /> mix 1020 TurboRFP-TA vector 8clonies insert together,and ligate to digested pLVX-Plus2 164&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Colony Screening <br /> <br /> Date: 2012-3-15 <br /> <br /> Make miniprep and digest with Xho1 + Xba1&lt;br&gt; <br /> <br /> [[Image:1009 digest detect 3-15-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> no insert <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> &lt;br&gt;<br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu