Paul.xu at 16:17, 25 March 2012

2012-03-25T16:17:16Z

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== '''Cloning of 1020-TurboRFP-TA vector''' ==

'''Purpose:''' Cloning of 1020-TurboRFP-TA vector

'''Date: '''2012-3-5---3-7

'''Notebook:00'''

'''Page:'''

'''Clone ID:1020'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

Date:2012-2-17

[[Image:1008+1009 PCR 2012-2-10.JPG]] 

template:TurboRFP-1 to TurboRFP-20 mixture

primer F:TurboRFP-1

primer R:TurboRFP-20

 TA ligate method 

=== Colony Screening ===

Date: 2012-3-7

Make miniprep and digest with Xho1 + Xba1

[[Image:Turbo-RFP digest detect 3-7-2012.JPG]] 

clone 2,3,4,5,6,7,8,9 positive

Date: 2012-3-22

Make miniprep and digest with Xho1 /Xba1 /EcoR1 

[[Image:TurboRFP-TA digest detect 3-22-2012.JPG]] 

[[Image:1020-TurboRFP-TA_vector_EcoR1.jpg]] 

[[Image:1020-TurboRFP-TA_vector_Xho1_Xba1.jpg]]

== '''Result intepretation/Summary''' ==

* 

== '''Conclusion''' ==

 

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[[Category:Cloning]]

1020-TurboRFP-TA vector - Revision history

Paul.xu at 16:17, 25 March 2012