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1028-pET28b-LambdaGam - Revision history
2026-10-18T05:16:05Z
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Xiaojing.Wang at 19:06, 4 May 2012
2012-05-04T19:06:20Z
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<p><b>New page</b></p><div>Cloning of pET28b-LambdaGam(1028)<br>Purpose: to clone pET28b-LambdaGam<br>Date: 2012-4-26 to 2012-4-<br>Notebook: Xiaojing Wang<br>Page: 004233<br>Clone ID: 1028<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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subclone from 1071 pET30b-lambda-gama using Nde1 + Xho1, vector is pET28b<br> <br />
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[[Image:1028 insert 4.26.2012.JPG|119x326px|1028 insert 4.26.2012.JPG]]<br> <br />
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[[Image:1103 1028 v digest 4.26.2012.JPG]]<br> <br />
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Ligate, transform top10 <br />
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Select 5 clones to mini-prep<br>1028 plasmids are digested by Nde1 + Xho1<br> <br />
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[[Image:1028 digest 4.30.2012.JPG]]<br> <br />
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[[Image:1028-2 digest 5.3.2012.JPG]]<br />
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1028-2 may be correct. <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang