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1029-pHisGST - Revision history
2026-10-18T04:05:03Z
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Xiaojing.Wang at 17:58, 26 March 2012
2012-03-26T17:58:16Z
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<p><b>New page</b></p><div>Cloning of pHisGST (1029)<br>Purpose: to clone pHisGST <br>Date: 2012-3-21 to 2012-3-23<br>Notebook: Xiaojing Wang<br>Page: 004203<br>Clone ID: 1029<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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digest pGST 455 with Nde1 + EcoR1, digest pET28b with Nde1 + EcoR1.<br> <br />
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[[Image:1029 digest 3.21.2012.JPG]]<br> <br />
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ligate, transform top10<br> <br />
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Select 4 clones to mini-prep<br>1029 plasmids are digested by Nde1 + EcoR1 <br> <br />
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[[Image:1029 digest 3.26.2012.JPG]] <br />
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clone 1029-1,-2,-3,-4 may be correct. <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang