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1031-pSpeedET-wt-Taq - Revision history
2026-10-18T03:31:50Z
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Xiaojing.Wang at 17:59, 26 March 2012
2012-03-26T17:59:26Z
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<p><b>New page</b></p><div>Cloning of pSpeedET-wt-Taq (1031)<br>Purpose: to clone pSpeedET-wt-Taq<br>Date: 2012-3-21 to 2012-3-23<br>Notebook: Xiaojing Wang<br>Page: 004203<br>Clone ID: 1031<br>Experimenters: Xiaojing Wang<br>Results <br />
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Digest 462 Wt-Taq-XuYan with Nco1 + Xba1, digest pSpeedET 337 with Nco1 + Xba1. <br> <br />
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[[Image:1031 digest 3.21.2012.JPG]]<br> <br />
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Ligate, transform top10 <br />
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Select 4 clones to mini-prep<br>1031 plasmids are digested by Nco1 + Xba1 <br> <br />
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[[Image:1031 digest 3.26.2012.JPG]]<br> <br />
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Clone 1031-1,-2,-3 may be correct. <br> <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang