https://eln.excellgen.com/index.php?action=history&feed=atom&title=1032-pET6XHis-sso7d-wt-Taq 2026-10-18T03:31:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1032-pET6XHis-sso7d-wt-Taq&diff=3742&oldid=prev 2012-04-03T20:20:51Z

<p></p> <p><b>New page</b></p><div>Cloning of pET6XHis-sso7d-wt-Taq (1032)&lt;br&gt;Purpose: to clone pET6XHis-sso7d-wt-Taq&lt;br&gt;Date: 2012-3-26 to 2012-3-30&lt;br&gt;Notebook: Xiaojing Wang&lt;br&gt;Page: 004206&lt;br&gt;Clone ID: 1032&lt;br&gt;Experimenters: Xiaojing Wang&lt;br&gt;Results &lt;br&gt; <br /> <br /> [[Image:1032 PCR 3.27.2012.JPG]]&lt;br&gt; <br /> <br /> The bands show PCR product of 1032, &lt;br&gt; <br /> <br /> sso7d-Nde1-F&lt;br&gt;aca tac CAT ATG gGC AGC AGC CACCATCATCATCATCAT acg gtc aaa ttc aag tac aaag <br /> <br /> sso7d-EcoR1-R <br /> <br /> (G AAA AAA GGC GGT GGT GGC GAA TTC agagta)&lt;br&gt;tactctGAATTCGCCACCACCGCCTTTTTTC&lt;br&gt; <br /> <br /> Digest result: &lt;br&gt; <br /> <br /> [[Image:1032 P V digest 3.28.2012.JPG]]&lt;br&gt; <br /> <br /> digest PCR product and vector with Nde1+EcoR1, run gel <br /> <br /> the left bands show 1032 insert, the right bands show 512 vector <br /> <br /> gel purify, ligate 2 hours, transform Top10,K+ &lt;br&gt; <br /> <br /> Colony Screening &lt;br&gt; <br /> <br /> [[Image:1032 digest 3.30.2012.JPG]]&lt;br&gt; <br /> <br /> 1032-1,-2,-3 may be correct. <br /> <br /> Select 1032-3 to sequence.&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang