https://eln.excellgen.com/index.php?action=history&feed=atom&title=1033-pGST-DNase1 2026-10-18T11:52:10Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1033-pGST-DNase1&diff=3665&oldid=prev 2012-03-27T21:35:20Z

<p></p> <p><b>New page</b></p><div>Cloning of pGST-DNase1 (1033)&lt;br&gt;Purpose: to clone pGST-DNase1&lt;br&gt;Date: 2012-3-21 to 2012-3-23&lt;br&gt;Notebook: Xiaojing Wang&lt;br&gt;Page: 004203&lt;br&gt;Clone ID: 1033&lt;br&gt;Experimenters: Xiaojing Wang&lt;br&gt;Results <br /> <br /> digest pGST 455 vector with EcoR1 + Xho1, digest &lt;strike&gt;pGST-DNase1&lt;/strike&gt; &lt;font color=&quot;red&quot;&gt; pET28-DNase1 202 &lt;/font&gt;with EcoR1 + Xho1-- insert&lt;br&gt; <br /> <br /> [[Image:1033 digest 3.21.2012.JPG]]&lt;br&gt; <br /> <br /> Ligate, transform top10 <br /> <br /> Select 4 clones to mini-prep&lt;br&gt;1033 plasmids are digested by EcoR1 +Xho1&lt;br&gt; <br /> <br /> [[Image:1033 digest 3.26.2012.JPG]]&lt;br&gt; <br /> <br /> Clone 1033-1,-2,-3,-4 may be correct. &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu