https://eln.excellgen.com/index.php?action=history&feed=atom&title=1034-pET8X-UDG 1034-pET8X-UDG - Revision history 2026-10-18T11:23:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1034-pET8X-UDG&diff=3732&oldid=prev Paul.xu at 19:39, 2 April 2012 2012-04-02T19:39:15Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1034-pET8X-UDG&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1034-pET8X-UDG <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-3-26--2012 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1034&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-3-26 <br /> <br /> [[Image:1034 PCR 3-26-2012.JPG]] <br /> <br /> gene synthesis:UDG-1 to UDG-22&lt;br&gt; [[Image:1009+1017+1023+1034 clone 3-27-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 335px; height: 99px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 7&lt;br&gt; <br /> | band 8&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 286, pET8XHis&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-3-28 <br /> <br /> PCR screen <br /> <br /> [[Image:1023+1034 PCR screen 3-28-2012.JPG]] <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1034-pET8X-UDG&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1034-pET8X-UDG <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-3-30--2012 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1034&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2012-3-26 <br /> <br /> [[Image:1034 PCR 3-26-2012.JPG]] <br /> <br /> gene synthesis:UDG-1 to UDG-22&lt;br&gt; &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 335px; height: 99px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 7&lt;br&gt; <br /> | band 8&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR &lt;br&gt; <br /> | 286, pET8XHis&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012- 4-2&lt;br&gt;<br /> <br /> miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1023+1034digest_detect_4-2-2012.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu