Paul.xu at 19:14, 6 April 2012

2012-04-06T19:14:12Z

New page

== '''Cloning of 1036-pET30-RecA''' ==

'''Purpose:''' Cloning of 1036-pET30-RecA

'''Date: '''2012-4-2---2012-4-6

'''Notebook:0'''

'''Page:'''

'''Clone ID:1036'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

[[Image:1036_PCR_4-2-2012.JPG]] 

PCR using Top10 genomic DNA RecA-Nde1-F actagcgCATATGGCTATCGACGAAAACAAAC

RecA-Xho1-R (GCAGAAACTAACGAAGATTTTCTCGAG cgaggc) gcctcgCTCGAGAAAATCTTCGTTAGTTTCTGC 

digest: 

[[Image:1023+1036_clone_4-4-2012.JPG]] 

{| cellspacing="1" cellpadding="1" border="1" style="width: 290px; height: 64px;"
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| band 3 
| band 4 
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| PCR product 
| pET30 
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| enzymes 
| colspan="2" |                   Nde1 + Xho1 
|}

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=== Colony Screening ===

Date: 2012-4-6

Make miniprep and digest with Nde1 + Xho1

[[Image:1023+1036_digest_detect_4-6-2012.JPG]]

clone 1,2,3,4,5,6 may be right, need to sequence

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

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[[Category:Cloning]]

1036-pET30-RecA - Revision history

Paul.xu at 19:14, 6 April 2012