https://eln.excellgen.com/index.php?action=history&feed=atom&title=1037_pET30b-T7ligase 1037 pET30b-T7ligase - Revision history 2026-10-18T02:20:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1037_pET30b-T7ligase&diff=3618&oldid=prev Paul.xu at 15:38, 25 March 2012 2012-03-25T15:38:00Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1037 pET30b-T7ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1037 T7 ligase by gene synthesis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-3-25- <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1037&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Gene synthesis&lt;br&gt; ===<br /> <br /> Date:2012-3-25 &lt;br&gt; [[Image:T7 ligase 1st PCR 2012 3 25.jpg]]&lt;br&gt; <br /> <br /> 1: first fragment, oligo 1-14 <br /> <br /> 2: 2nd fragment, oligo 15-30 <br /> <br /> [[Image:T7 ligase 2nd PCR 2012 3 25.jpg]] <br /> <br /> [[Image:T7 ligase 2nd PCR2 2012 3 25.jpg]] <br /> <br /> Lane 6 &amp;amp; 7: 2nd round PCR using combined fragments (1 &amp;amp;2) <br /> <br /> [[Image:T7_ligase_digestion_2012_3_25.jpg]]&lt;br&gt; <br /> <br /> 1: Nde1+ Xho1 digestion of gene synthesis fragment<br /> <br /> 2: pET30 digestion with Nde1 + Xho1.<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu