https://eln.excellgen.com/index.php?action=history&feed=atom&title=1044_pET28b-T4-DNA_Pol
1044 pET28b-T4-DNA Pol - Revision history
2026-10-18T02:19:22Z
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https://eln.excellgen.com/index.php?title=1044_pET28b-T4-DNA_Pol&diff=8009&oldid=prev
Paul.xu at 21:32, 28 January 2013
2013-01-28T21:32:07Z
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<p><b>New page</b></p><div>== '''Cloning of 1044 pET28b-T4-DNA Pol''' ==<br />
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'''Purpose:''' Cloning of 1044 pET28b-T4-DNA Pol <br />
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'''Date: '''Jan , 2013<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: 1044''' <br />
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'''Experimenters: '''Shanyi <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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PCR<br> <br />
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[[Image:1044 pcr 2013 1 11.JPG]] <br />
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50oc 30s, 65oc 5min, 30cycle<br><br />
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[[Image:Digest for 1044 1049 1122 1123 1 16 2013.JPG]] <br />
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=== Colony screen ===<br />
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[[Image:1027-2 1044DIGEST.JPG]] <br />
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<br> <br />
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== '''Cloning of 1044 pET28b-T4-DNA Pol''' ==<br />
<br />
'''Purpose:''' Cloning of 1044 pET28b-T4-DNA Pol <br />
<br />
'''Date: '''Jan , 2013<br> <br />
<br />
'''Notebook:&nbsp;''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID: 1044''' <br />
<br />
'''Experimenters: '''Shanyi <br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
PCR<br> <br />
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[[Image:1044_1045_1538-1pcr1_15_2013.JPG]]<br><br />
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use first round pcr as template, 52oc 30s,72oc 3min,29cycle.<br><br />
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[[Image:DIGEST_FOR_1044_1538_1_23_2013.JPG]]<br><br />
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digest with NheI and Sal1<br> <br />
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=== Colony screen ===<br />
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digest with NheI and Sal1 <br><br />
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&nbsp;[[Image:1044digest_1_28_2013.JPG ]]<br><br />
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<br><br />
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[[Category:Cloning]]</div>
Paul.xu