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1060-pLVX-CAG-MCS-IRES-mCherry - Revision history
2026-10-18T09:40:10Z
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Xiaojing.Wang at 14:19, 18 April 2012
2012-04-18T14:19:34Z
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<p><b>New page</b></p><div>Cloning of pLVX-CAG-MCS-IRES-mCherry(1060)<br>Purpose: to clone pLVX-CAG-MCS-IRES-mCherry<br>Date: 2012-4-2 to 2012-4-4<br>Notebook: Xiaojing Wang<br>Page: 004211,004215<br>Clone ID: 1060<br>Experimenters: Xiaojing Wang<br>Results <br />
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Digest pCAGMKOSiE, 313(13#) with Nde1 + EcoR1, digest pLVXPlus3-IRES-mCherry,531 with Nde1 + EcoR1.<br> <br />
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[[Image:1060 i v digest 4.2.2012.JPG]]<br> <br />
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Band 1 is 313 digested by Nde1.<br> <br />
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Band 2 is 313 digested by EcoR1.<br> <br />
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Band 3 is 313 digested by Nde1+EcoR1.<br> <br />
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Ligate, transform STBL3,A <br />
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Select 5 clones to mini-prep<br>1060 plasmids are digested by Nde1 + EcoR1 <br> <br />
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[[Image:1060 digest 4.4.2012.JPG]]<br> <br />
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Clone 1060-4,-5 may be correct. <br> <br />
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Select 1060-4 to sequence. <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang