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1061-pLVX-CAG-MCS-IRES-EGFP - Revision history
2026-10-18T14:43:17Z
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Xiaojing.Wang at 17:09, 19 April 2012
2012-04-19T17:09:05Z
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<p><b>New page</b></p><div>Cloning of pLVX-CAG-MCS-IRES-EGFP(1061)<br>Purpose: to clone pLVX-CAG-MCS-IRES-EGFP<br>Date: 2012-4-12 to 2012-4-18<br>Notebook: Xiaojing Wang<br>Page: 004219<br>Clone ID: 1061<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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[[Image:I v digest 4.12.2012.JPG]] <br />
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cut out IRES-EGFP from pLVX-IRES-EGFP 528 using Xho1 + Mlu1, gel purify insert<br>Digest pLVX-CAG-MCS-IRES-mCherry, 1060 with Xho1 + Mlu1, gel purify the vector<br>ligate . IRES-EGFP to pLVX-CAG-MCS<br> <br />
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Select 6 clones to mini-prep<br>1061 plasmids are digested by Xho1 + Mlu1 <br> <br />
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[[Image:1061 digest 4.17.2012.JPG]]<br> <br />
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Clone 1061-1,-2,-3,-4,-6 may be correct. <br />
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Select 1061-1,-2,-3,-4,-6 to transfect. <br> <br />
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[[Image:1061 transfection 4.19.2012.jpg]]<br> <br />
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Clone 1061-1,-2,-3,-4,-6 may be correct. <br><br />
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[[Category:Cloning]]</div>
Xiaojing.Wang