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1064-pLex-CMV5-CuO-Bcl-2 - Revision history
2026-10-18T02:19:45Z
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Xiaojing.Wang at 15:26, 3 April 2012
2012-04-03T15:26:30Z
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<p><b>New page</b></p><div>Cloning of pLex-CMV5-CuO-Bcl-2 (1064)<br>Purpose: to clone pLex-CMV5-CuO-Bcl-2<br>Date: 2012-3-27 to 2012-3-30<br>Notebook: Xiaojing Wang<br>Page: 004207-004208<br>Clone ID: 1064<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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Gateway LR reaction, 1019 pDONR-Bcl-2 + 456 pLex-CMV-CuO-Dest<br> <br />
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Transformation, STBL3,A+ <br />
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Select 4 clones to mini-prep. <br />
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[[Image:1064 digest1 3.29.2012.JPG]] <br />
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[[Image:1064 digest2 3.29.2012.JPG]] <br />
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1064-1,-3,-4 may be correct. <br />
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Select 1064-1 to sequence. <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang