https://eln.excellgen.com/index.php?action=history&feed=atom&title=1070-pAd52-CMV5-Cuo-IRES-EGFP 1070-pAd52-CMV5-Cuo-IRES-EGFP - Revision history 2026-10-18T04:25:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1070-pAd52-CMV5-Cuo-IRES-EGFP&diff=4022&oldid=prev Paul.xu at 18:20, 30 April 2012 2012-04-30T18:20:46Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1070-pAd52-CMV5-Cuo-IRES-EGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1070-pAd52-CMV5-Cuo-IRES-EGFP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-26---2012-4-30<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1070&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1068+1070+1090+1092 clone 4-26-2012.JPG]] <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 444px; height: 115px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 3&lt;br&gt; <br /> | band 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 533 pLVX-Cre-IRES-EGFP&lt;br&gt; <br /> | pAd52-CMV5-Cuo-MCS3, 496&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | Xho1 +Mlu1&lt;br&gt; <br /> | Sal1 +Mlu1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-4-30<br /> <br /> Make miniprep and digest with EcoR1 + Mlu1&lt;br&gt; <br /> <br /> [[Image:1070+1090_digest_detect_4-30-2012.JPG]]<br /> <br /> need to transfection and sequence<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu