Paul.xu at 20:11, 20 April 2012

2012-04-20T20:11:58Z

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== '''Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc''' ==

'''Purpose:''' Cloning of 1079-pLVX-loxP-sfGFP-loxP-Gluc

'''Date: '''2012-4-10--2012-4-15

'''Notebook:0'''

'''Page:'''

'''Clone ID:1079'''

'''Experimenters:''' yingying

== '''Results''' ==

=== cloning ===

Date:2012-4-10

 [[Image:1079 PCR 4-10-2012.JPG]]

template:300pLentiGP-Gluc-PKAalpha 

Primer-F Gluc-Xho1-F TAcAG G ctcgag gcc acc ATGGGAGTCAAAGTTCTGTTTG Primer-R Gluc-Xba1-R (CAAGGGGGCCGGTGGTGACTAA TCT AGA tatgcat) 

[[Image:1079 clone 4-11-2012.JPG]] 

{| cellspacing="1" cellpadding="1" border="1" style="width: 332px; height: 80px;"
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| band 1 
| band 2 
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| PCR product 
| 1008pLVX-loxP-sfGFP-loxP 
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| enzymes 
| colspan="2" | Xho1+Xba1 
|}

 

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=== Colony Screening ===

Date: 2012-4-13

Make miniprep and digest with Xho1+Xba1

[[Image:1079 digest detect 4-13-2012.JPG]]

need to sequence

 

== '''Result intepretation/Summary''' ==

* 

== '''Conclusion''' ==

 

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[[Category:Cloning]]

1079-pLVX-loxP-sfGFP-loxP-Gluc - Revision history

Paul.xu at 20:11, 20 April 2012