https://eln.excellgen.com/index.php?action=history&feed=atom&title=1080-pBad24-RNAPol 1080-pBad24-RNAPol - Revision history 2026-10-18T11:51:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1080-pBad24-RNAPol&diff=3983&oldid=prev Paul.xu at 18:19, 25 April 2012 2012-04-25T18:19:56Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1080-pBad24-RNAPol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1080-pBad24-RNAPol <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-20---2012-4-25<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1080&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1080 PCR 2012-4-20.JPG]]&lt;br&gt; <br /> <br /> PCR using 1method: T7 phage DNA;&amp;nbsp; 2method: Nico21(DE3) cell genomic DNA&amp;nbsp; as template. <br /> <br /> T7RNAPol-EcoR1-F&lt;br&gt; EcoR1&lt;br&gt;acgaca GAATTC ATG AACACGATTAACATCGCTAAGAAC <br /> <br /> T7RNAPol-HindIII-R&lt;br&gt;(GAGTCGGACTTCGCGTTCGCG CAT CAT CAT CAT CAT CAC TAA AAGCTT atacta)&lt;br&gt; [[Image:1080 clone 4-23-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 348px; height: 82px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pBad24 545&lt;br&gt;<br /> |-<br /> | enzymes &lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; EcoR1 + HindIII&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-4-25 <br /> <br /> Make miniprep and digest with EcoR1 + HindIII <br /> <br /> [[Image:1080 digest detect 4-25-2012.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu