https://eln.excellgen.com/index.php?action=history&feed=atom&title=1095-pET28b-T7Exo 2026-10-18T03:29:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1095-pET28b-T7Exo&diff=6930&oldid=prev 2012-11-08T20:10:56Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1095-pET28b-T7Exo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1095-pET28b-T7Exo <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-4-13---2012-4-19 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1095&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1017+1095 PCR 4-13-2012.JPG]]&lt;br&gt; <br /> <br /> template: PCR using T7 Phage DNA <br /> <br /> T7_Exo_Nde1-F&lt;br&gt;cacagg cat atg gcc atggcacttcttgaccttaaac <br /> <br /> T7_exo-Xho1_R&lt;br&gt;(gagattta cctgtggaga ccgtag ctcgag actagc) <br /> <br /> gctagtctcgagctacggtctccacaggtaaatctc <br /> <br /> [[Image:537+1095 clone 4-16-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 3&lt;br&gt; <br /> | band 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pET28b&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-4-19 <br /> <br /> Make miniprep and digest with Nde1+Xho1 <br /> <br /> [[Image:1092+1095 digest detect 4-19-2012.JPG|385x215px|1092+1095 digest detect 4-19-2012.JPG]][[Image:495+1092 digest detect 4-19-2012.JPG|132x217px|495+1092 digest detect 4-19-2012.JPG]]&lt;br&gt; clone 1 3 4 7 may be right, need to sequence&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of 1095-pET28b-T7Exo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1095-pET28b-T7Exo <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-10-19---2012-10-26 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1095&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:240+1095+1248 PCR 10-19-2012.JPG]]&lt;br&gt; <br /> <br /> template: PCR using T7 Phage DNA <br /> <br /> T7_Exo_Nde1-F&lt;br&gt;cacagg cat atg gcc atggcacttcttgaccttaaac <br /> <br /> T7_exo-Xho1_R&lt;br&gt;(gagattta cctgtggaga ccgtag ctcgag actagc) <br /> <br /> gctagtctcgagctacggtctccacaggtaaatctc <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:240+1095 clone 10-23-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 323px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 2&lt;br&gt; <br /> | band &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1+Xho1&lt;br&gt;<br /> |}<br /> <br /> [[Image:240+1095+1420 clone 10-24-2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;386&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | lane2&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1027,pET28b-LambdaBet&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | Nde1+Xho1&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-10-26 <br /> <br /> Make miniprep and digest with Nde1+Xho1 <br /> <br /> [[Image:240+1095 digest detect 10-26-2012.JPG]] <br /> <br /> &lt;br&gt;<br /> <br /> 2012-11-8&lt;br&gt;<br /> <br /> PCR Screening:&amp;nbsp; T7_Exo_Nde1-F+T7 term&lt;br&gt;<br /> <br /> [[Image:1095_pcr_screening_11-8-2012.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu