https://eln.excellgen.com/index.php?action=history&feed=atom&title=1096-pET28b-T3-Ligase 2026-10-18T04:25:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1096-pET28b-T3-Ligase&diff=7298&oldid=prev 2012-12-03T20:45:17Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1096-pET28b-T3-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1096-pET28b-T3-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-11-19--2012-11-27 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1096&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Gene synthesis of Fragment1 and 2.&lt;br&gt; <br /> <br /> [[Image:1096 PCR1+PCR2 11-20-2012.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Join them by 2nd round PCR&lt;br&gt; <br /> <br /> [[Image:1096+1463 PCR 11-20-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:240+1096+1463 clone 11-20-2012.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;338&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1027,pET28b-LambdaBet&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; Nde1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-11-26 <br /> <br /> mini prep and digest with Nde1+Xho1&lt;br&gt; <br /> <br /> [[Image:1096 digest detect 11-26-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> T3 1-1 +T7 Term <br /> <br /> [[Image:1096 PCR screen 11-27-2012.JPG]] <br /> <br /> clone 2,3,4,6,7,8 right ,need to sequence <br /> <br /> &lt;br&gt; <br /> <br /> 2012-11-28&lt;br&gt; <br /> <br /> mini prep and digest with Nde1+Xho1&lt;br&gt; <br /> <br /> [[Image:1096 digest detect 11-28-2012.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt; <br /> <br /> {| width=&quot;389&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

Paul.xu