https://eln.excellgen.com/index.php?action=history&feed=atom&title=10_X_PBS 2026-10-18T07:25:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=10_X_PBS&diff=3446&oldid=prev 2012-03-03T22:50:26Z

<p></p> <p><b>New page</b></p><div>== Protocol 1&lt;br&gt; ==<br /> <br /> 22.8 g NaH&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt; (mw=120; 0.038M)&amp;nbsp; or 26.2g NaH&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt;(H2O) (mw=137.99; 0.038M) (Note: monobasic)&lt;br&gt;115g Na&lt;sub&gt;2&lt;/sub&gt;HPO&lt;sub&gt;4&lt;/sub&gt; (mw=141.96; 0.162M) (Note: dibasic)&lt;br&gt;438.4g NaCl <br /> <br /> Make it up <br /> <br /> Add NaH&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt; and Na&lt;sub&gt;2&lt;/sub&gt;HPO&lt;sub&gt;4&lt;/sub&gt; and NaCl to 4500 ml dH2O.&lt;br&gt; <br /> <br /> &lt;br&gt;Adjust pH to 7.4 by adding dilute (1N) HCl if necessary.&lt;br&gt; <br /> <br /> &lt;br&gt;Bring the final volume to 5000 ml with water.&lt;br&gt; <br /> <br /> &lt;br&gt;Dilute 1:10 for final concentration.&lt;br&gt; <br /> <br /> == Protocol 2 ==<br /> <br /> 137 mM NaCl&lt;br&gt; 2.7 mM KCl&lt;br&gt; 100 mM Na&lt;sub&gt;2&lt;/sub&gt;HPO&lt;sub&gt;4&lt;/sub&gt;&lt;br&gt; 2 mM KH&lt;sub&gt;2&lt;/sub&gt;PO&lt;sub&gt;4&lt;/sub&gt; <br /> <br /> To make 10X PBS Buffer, dissolve the following reagents in 800 ml ultrapure water.&lt;br&gt;NaCl 80 g&lt;br&gt;KCl&amp;nbsp;&amp;nbsp; 2 g&lt;br&gt;Na2HPO4 14.4 g&lt;br&gt;KH2PO4 2.4 g&lt;br&gt; <br /> <br /> Adjust pH of PBS Buffer Solution to 7.4 with HCl.&lt;br&gt;Bring volume to 1 liter, autoclave or sterilize by filtration.&lt;br&gt;&lt;br&gt; <br /> <br /> == Protocol 3 ==<br /> <br /> A 10 liter stock of 10x PBS can be prepared by dissolving 800 g NaCl, 20 g KCl, 144 g&lt;br&gt;Na2HPO4 · 2H2O and 24 g KH2PO4 in 8 L of distilled water, and topping up to 10 L. The&lt;br&gt;pH is ~6.8, but when diluted to 1x PBS it should change to 7.4. When making buffer&lt;br&gt;solutions, it is good practice to always measure the pH directly using a pH meter. If&lt;br&gt;necessary, pH can be adjusted using hydrochloric acid or sodium hydroxide.&lt;br&gt;On dilution, the resultant 1x PBS should have a final concentration of 137 mM NaCl, 10&lt;br&gt;mM Phosphate, 2.7 mM KCl, and a pH of 7.4.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == PBS 20x stock ==<br /> <br /> &lt;br&gt;Potassium chloride 4 g&lt;br&gt;NaCl 160 g&lt;br&gt;Potassium phosphate monobasic 4 g&lt;br&gt;Sodium phosphate dibasic (7•H2O) DI 43.2 g&lt;br&gt;&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Buffer_Recipe]]</div>

Paul.xu