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1102-pLVX-CMV5-MCS-IRES-mCherry - Revision history
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Xiaojing.Wang at 17:49, 26 April 2012
2012-04-26T17:49:45Z
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<p><b>New page</b></p><div>Cloning of pLVX-CMV5-MCS-IRES-mCherry(1102)<br>Purpose: to clone pLVX-CMV5-MCS-IRES-mCherry<br>Date: 2012-4-18 to 2012-4-23<br>Notebook: Xiaojing Wang<br>Page: 004227<br>Clone ID: 1102<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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digest pLex-CMV5-CuO, 448 with Nde1 + EcoR1--&gt; 1kb CMV5 promoter<br> <br />
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digest pLVX-CAG-MCS-IRES-mCherry, 1060 with Nde1 + EcoR1--&gt;vector<br> <br />
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[[Image:I v digest N2 4.19.2012.JPG|132x330px|I v digest N2 4.19.2012.JPG]]<br> <br />
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ligate and transform STBL3<br> <br />
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Select 6 clones to mini-prep<br>1102 plasmids are digested by Nde1 + EcoR1<br> <br />
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[[Image:1102 digest 4.23.2012.JPG]]<br> <br />
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Clone 1102-2,-3,-4,-5 may be correct. <br><br> <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang