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1103-pLVX-CMV5-MCS2-IRES-mCherry - Revision history
2026-10-18T09:55:09Z
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Xiaojing.Wang at 19:19, 30 April 2012
2012-04-30T19:19:54Z
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<p><b>New page</b></p><div>Cloning of pLVX-CMV5-MCS2-IRES-mCherry(1103)<br>Purpose: to clone pLVX-CMV5-MCS2-IRES-mCherry<br>Date: 2012-4-26 to 2012-4-30<br>Notebook: Xiaojing Wang<br>Page: 004232<br>Clone ID: 1103<br>Experimenters: Xiaojing Wang<br>Results <br> <br />
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digest vector (pLVX-CMV5-MCS-IRES-mCherry, 1102) with BamH1 + Spe1, <br> <br />
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[[Image:1103 1028 v digest 4.26.2012.JPG]]<br> <br />
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ligate to annealed oligos: <br />
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pLVX-CMV5-MCS2-T <br />
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pLVX-CMV5-MCS2-B ,transform STBL3<br> <br />
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Select 6 clones to mini-prep<br>1103 plasmids are digested by Nde1 + Pac1<br> <br />
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[[Image:1103 digest 4.30.2012.JPG]]<br> <br />
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1103-1~-6 may be correct.<br> <br />
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[[Category:Cloning]]</div>
Xiaojing.Wang