https://eln.excellgen.com/index.php?action=history&feed=atom&title=1114-pLC6H-TneSSB 1114-pLC6H-TneSSB - Revision history 2026-10-18T10:35:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1114-pLC6H-TneSSB&diff=11238&oldid=prev Paul.xu: Created page with "== '''Cloning of 1114-pLC6H-TneSSB''' == '''Purpose:''' Cloning of 1114-pLC6H-TneSSB '''Date: '''2014-2-12---2014-2-14 '''Notebook:0''' '''Page:''' '''Clone ID:1114''' ..." 2014-02-14T21:30:13Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1114-pLC6H-TneSSB&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1114-pLC6H-TneSSB &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1114&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1114-pLC6H-TneSSB&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1114-pLC6H-TneSSB<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-2-12---2014-2-14<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1114&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:715_718_1114_1142_1322_1972_2015PCR_1-15-2014.jpg]]<br /> <br /> gene synthesis&lt;br&gt;<br /> <br /> TneSSB-1 to TneSSB-12&amp;nbsp; &lt;br&gt;<br /> <br /> [[Image:1114_1142_1940_2016clone_2-12-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 2030 pLC6H-sfGFP&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 +Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-2-14<br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1114_1142digest_2-14-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu