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1121 pET28b-Exo-1 - Revision history
2026-10-18T08:19:29Z
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Xiaojing.Wang at 19:07, 27 July 2012
2012-07-27T19:07:01Z
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<p><b>New page</b></p><div>== '''Cloning of 1121 pET28b-Exo-1''' ==<br />
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'''Purpose:''' Cloning of 1121 pET28b-Exo-1<br />
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'''Date: '''July 23, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1121 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date:&nbsp;July 24, 2012<br> <br />
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[[Image:0724 Amp.JPG]]<br> <br />
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See lane 2, expected size 1428 bp<br> <br />
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PCR from MG1665 genomic DNA <br />
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Exo-I-Nde1-F<br>Nde1<br>cagacaCAT ATGATGAATGACGGTAAGCAAC <br />
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Exo-I-Xho1-R<br>(GGCAGTACGCGGAAGAGATTGTC CTCGAG cagatc)<br>gatctgCTCGAGGACAATCTCTTCCGCGTACTGCC<br> <br />
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Cut out band, purify, digest with RE&nbsp;Ndel and Xho1, with vector pET28b <br />
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[[Image:0726_Digestion.JPG]]<br />
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See lanes 6 (insert)&nbsp;and 7 (vector), cut out bands, purify and ligate together and transform<br />
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=== <u></u>Colony Screening<br> ===<br />
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Date:&nbsp;July 27, 2012<br />
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[[Image:0727_Digestion.JPG]]<br />
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Digested MiniPrep with RE&nbsp;Nde1 and Xho1, see lanes 13-18<br />
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All samples are positive for insert, submitted sample 2 for sequencing<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Xiaojing.Wang