https://eln.excellgen.com/index.php?action=history&feed=atom&title=1122_pET28b-T4RNA-Lig2-truncated 1122 pET28b-T4RNA-Lig2-truncated - Revision history 2026-10-18T02:16:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1122_pET28b-T4RNA-Lig2-truncated&diff=7901&oldid=prev Paul.xu at 22:25, 18 January 2013 2013-01-18T22:25:47Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1122 pET28b-T4RNA-Lig2-truncated&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1122 pET28b-T4RNA-Lig2-truncated <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Dec, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1122 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR, &lt;br&gt; 1049_1122_1530DIGEST.JPG‎ T4RNA-lig-Nde1-F&lt;br&gt;T4RNA-lig-2-Xho1-R&lt;br&gt; <br /> <br /> [[Image:1044 1122 1123pcr.JPG]]&lt;br&gt; <br /> <br /> Nde1 + Xho1.&lt;br&gt; <br /> <br /> [http://www.excellgen.net/eln/images/e/e4/1049_1122_1530DIGEST.JPG 1049_1122_1530DIGEST.JPG]‎[[Image:V12 1122pcr 1505digest.JPG]]&lt;br&gt; <br /> <br /> === Clone screen ===<br /> <br /> [[Image:1122digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1122 pET28b-T4RNA-Lig2-truncated&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1122 pET28b-T4RNA-Lig2-truncated <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Dec, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1122 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR, &lt;br&gt; <br /> <br /> [[Image:1045 1049 1122 1123pcr.JPG]]&lt;br&gt; <br /> <br /> [[Image:Digest for 1044 1049 1122 1123 1 16 2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Screening ===<br /> <br /> [[Image:1049_1122_1530DIGEST.JPG‎]]<br /> <br /> [[Image:1122 1123DIGEST.JPG]] <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu