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1133 pET28b-M1wt - Revision history
2026-10-18T06:28:22Z
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Excellgen at 16:12, 5 February 2016
2016-02-05T16:12:56Z
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<p><b>New page</b></p><div>5/5/2012<br />
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PCR amplification, 25 ul, using Kapa HiFi<br />
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[[File:1133 PCR 5 5 2012.jpg|RTENOTITLE]][[File:1113 vector digestion 5 5 2012.jpg|RTENOTITLE]]<br />
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digest gel-purified PCR product and pET28b with Nde1 + Xho1<br />
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ligate and transform DH5alpha<br />
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Select 6 clones to mini-prep<br/>1133 plasmids are digested by Nde1+Xho1<br />
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[[File:1133 digest 5.8.2012.JPG|RTENOTITLE]]<br />
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--[[User:Excellgen|Excellgen]] ([[User talk:Excellgen|talk]]) 11:12, 5 February 2016 (EST)<br />
[[Category:Cloning]]</div>
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