https://eln.excellgen.com/index.php?action=history&feed=atom&title=1134_pT7-Ecoli-Ligase 1134 pT7-Ecoli-Ligase - Revision history 2026-10-18T17:27:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1134_pT7-Ecoli-Ligase&diff=7032&oldid=prev Paul.xu: Created page with "== '''Cloning of 1134 pT7-Ecoli-Ligase''' == '''Purpose:''' Cloning of 1134 pT7-Ecoli-Ligase '''Date: '''Nov 1, 2012<br> '''Notebook:&nbsp;''' '''Page:''' '''Clone ID: ..." 2012-11-14T19:18:27Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1134 pT7-Ecoli-Ligase&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1134 pT7-Ecoli-Ligase &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 1, 2012&lt;br&gt; &#039;&#039;&#039;Notebook: &#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID: ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1134 pT7-Ecoli-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1134 pT7-Ecoli-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;Nov 1, 2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1134&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:shanyi&#039;&#039;&#039; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning&lt;br&gt; ===<br /> <br /> Date:&amp;nbsp;Nov 1, 2012&lt;br&gt; <br /> <br /> &lt;br&gt;PCR,<br /> <br /> [[Image:1134_1426PCR_11_02_2012.JPG]]<br /> <br /> Cut out band and purified <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> Ligate , transform&amp;nbsp; <br /> <br /> === &lt;u&gt;&lt;/u&gt;Colony Screening&lt;br&gt; ===<br /> <br /> [[Image:Smn2--smn1digest_11_05-2012.JPG]]&lt;br&gt; <br /> <br /> digestion (Nde1 + Xho1)&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu