https://eln.excellgen.com/index.php?action=history&feed=atom&title=1135_p8H 2026-10-18T10:23:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1135_p8H&diff=4196&oldid=prev 2012-05-14T18:39:13Z

<p></p> <p><b>New page</b></p><div>Digest pET28b with Nco1 + Nde1&lt;br&gt;<br /> <br /> [[Image:1135 v digest 5.8.2012.JPG]]&lt;br&gt;<br /> <br /> ligate to annealed&lt;br&gt;Nco1-8XHis-Nde1-T&lt;br&gt;Nco1-8XHis-Nde1-B &lt;br&gt;<br /> <br /> 5/11/2012 <br /> <br /> Sequencing result: #1 is no good (both Nde1 + Nco1 sites are gone, which means the oligos are not annealed properly), so digest plasmid DNA with Nde1 + Sma1. Send clone #3 for sequencing <br /> <br /> [[Image:1035 Nde1 Sma1 5 12 2012.jpg]] <br /> <br /> 3/12/2012: Sequencing result: #3 is good. Both Nco1 and Nde1 are preserved, and 8XHis tag is added to the vector. <br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang