https://eln.excellgen.com/index.php?action=history&feed=atom&title=1137-1121-1291-1252-1206 1137-1121-1291-1252-1206 - Revision history 2026-10-18T02:18:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1137-1121-1291-1252-1206&diff=7497&oldid=prev Paul.xu at 16:19, 13 December 2012 2012-12-13T16:19:17Z <p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Protein Expression Screeening&#039;&#039;&#039;&lt;br&gt; <br /> <br /> Date:12-6-2012-12-7-2012&lt;br&gt; <br /> <br /> Experimentor: Xiuli&lt;br&gt; <br /> <br /> Description:&lt;br&gt; <br /> <br /> Clones:&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 446px; height: 204px;&quot;<br /> |-<br /> | 1137&lt;br&gt; <br /> | p8H-Exo-VIII&lt;br&gt;&amp;nbsp;Ecoli exonuclease VIII, RecE&lt;br&gt;<br /> |-<br /> | 1121&lt;br&gt; <br /> | pET28b-Exo-1&lt;br&gt;&amp;nbsp;Ecoli exonuclease I&lt;br&gt;<br /> |-<br /> | 1291&lt;br&gt; <br /> | pET30b-TAT-NLS-sso7d&lt;br&gt;&amp;nbsp;2 TAT, NLS fused to sso7d&lt;br&gt;<br /> |-<br /> | 1206&lt;br&gt; <br /> | pET28b-recT&lt;br&gt;<br /> |-<br /> | 1203&lt;br&gt; <br /> | pET42b-scIHF2 &lt;br&gt;<br /> |-<br /> | 1245&lt;br&gt; <br /> | pDB.His.PfuMBP.scIHF2 &lt;br&gt;<br /> |-<br /> | 1249&lt;br&gt; <br /> | pET-Taq_d289&lt;br&gt;<br /> |-<br /> | 1252&lt;br&gt; <br /> | pTXB1-hLysozyme&lt;br&gt;&amp;nbsp;human lysozyme&lt;br&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;&lt;br&gt;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Gel 1 (Supernant)&#039;&#039;&#039; <br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;559&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1137&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1121&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1291&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1252&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1206&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | p8H-Exo-VIII&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-Exo-1&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET30b-TAT-NLS-sso7d &lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-hLysozyme&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-recT&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1137-1121-1291-1252-1206 SUP.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Gel2 (Pellet)&#039;&#039;&#039;&lt;br&gt; <br /> <br /> <br /> <br /> {| width=&quot;559&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1137&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1121&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1291&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1252&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1206&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | p8H-Exo-VIII&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-Exo-1&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET30b-TAT-NLS-sso7d &lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-hLysozyme&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-recT&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; &lt;br&gt; [[Image:1137-1121-1291-1252-1206 pellet.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu