https://eln.excellgen.com/index.php?action=history&feed=atom&title=1140_1325_1225_1206_1203_1245_1249_1464 1140 1325 1225 1206 1203 1245 1249 1464 - Revision history 2026-10-18T12:19:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1140_1325_1225_1206_1203_1245_1249_1464&diff=7519&oldid=prev Paul.xu at 19:24, 13 December 2012 2012-12-13T19:24:57Z <p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Protein Expression Screeening&#039;&#039;&#039;&lt;br&gt; <br /> <br /> Date:&lt;br&gt; <br /> <br /> Experimentor: Xiuli&lt;br&gt; <br /> <br /> Description:&lt;br&gt; <br /> <br /> Clones:&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 446px; height: 204px;&quot;<br /> |-<br /> | 1140&lt;br&gt; <br /> | pTXB1-polyAPol, E coli E coli poly(A) polymerase I&lt;br&gt;<br /> |-<br /> | 1325&lt;br&gt; <br /> | pDBHisMBP-gpI3L, vaccinia virus I3L gene &lt;br&gt;<br /> |-<br /> | 1225&lt;br&gt; <br /> | pET28b-T7-Endo1, T7 endonuclease 1&lt;br&gt;<br /> |-<br /> | 1206&lt;br&gt; <br /> | pET28b-recT&lt;br&gt;<br /> |-<br /> | 1203&lt;br&gt; <br /> | pET42b-scIHF2 &lt;br&gt;<br /> |-<br /> | 1245&lt;br&gt; <br /> | pDB.His.PfuMBP.scIHF2 &lt;br&gt;<br /> |-<br /> | 1249&lt;br&gt; <br /> | pET-Taq_d289&lt;br&gt;<br /> |-<br /> | 1464&lt;br&gt; <br /> | pT7A3-T7 ligase&lt;br&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;&lt;br&gt;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Gel 1 (supernant)&#039;&#039;&#039; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1325&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1203&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1245&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1464&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | pDBHisMBP-gpI3L&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET42b-scIHF2 &lt;br&gt; <br /> | colspan=&quot;2&quot; | pDB.His.PfuMBP.scIHF2 &lt;br&gt; <br /> | colspan=&quot;2&quot; | pT7A3-T7 ligase&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | I&lt;br&gt; <br /> | Pellet&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:Expression screening 12 1 2012 1.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Gel 2&#039;&#039;&#039; (Supernant)&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> pET28b-recT <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1140&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1225&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1206&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1249&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1140&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-polyAPol&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-T7-Endo1&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-recT&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET-Taq_d289&lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-polyAPol&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt; <br /> | 12&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:Expression screening 12 1 2012 2.jpg]] <br /> <br /> &lt;br&gt; <br /> <br /> Gel-3 (pellet) <br /> <br /> pET28b-recT <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1140&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1225&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1206&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1249&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1249-2&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-polyAPol&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-T7-Endo1&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET28b-recT&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET-Taq_d289&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET-Taq_d289&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt; <br /> | 11&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:1140 1225 1206 1249 pellet.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;nbsp; Gel 4 (pellet)&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | M&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1325&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1203&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1245&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1140-2&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | pDBHisMBP-gpI3L&lt;br&gt; <br /> | colspan=&quot;2&quot; | pET42b-scIHF2 &lt;br&gt; <br /> | colspan=&quot;2&quot; | pDB.His.PfuMBP.scIHF2 &lt;br&gt; <br /> | colspan=&quot;2&quot; | pTXB1-polyAPol&lt;br&gt; <br /> | MBP&lt;br&gt;<br /> |-<br /> | Lane&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | 5&lt;br&gt; <br /> | 6&lt;br&gt; <br /> | 7&lt;br&gt; <br /> | 8&lt;br&gt; <br /> | 10&lt;br&gt;<br /> |-<br /> | Sample&lt;br&gt; <br /> | &lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I&lt;br&gt; <br /> | C&lt;br&gt; <br /> | I &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Image:1325 1203 1245 1140 pellet.jpg]] <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu