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1140 pTXB1-polyAPol - Revision history
2026-10-18T02:24:37Z
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Xiaojing.Wang at 19:20, 27 July 2012
2012-07-27T19:20:58Z
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<p><b>New page</b></p><div>== '''Cloning of 1140 pTXB1-polyAPol''' ==<br />
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'''Purpose:''' Cloning of 1140 pTXB1-polyAPol<br />
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'''Date: '''July 23, 2012<br> <br />
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'''Notebook:&nbsp;''' <br />
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'''Page:''' <br />
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'''Clone ID: '''1140 <br />
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'''Experimenters: '''Jennifer Truong <br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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Date:&nbsp;July 23, 2012<br><br />
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[[Image:1140_Amp.JPG]]<br><br />
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Expected size 1398 bp, see lanes 5 and 6<br><br />
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PCR from MG1665 genomic DNA<br />
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polyAPol-Nde1-F<br>cgagca CATATGatttttacccgagtcgctaatttttg<br />
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polyAPol-Xho1-R<br>(caccacgtcgtgagggtaccgca CTCGAG atacta)<br>tagtatCTCGAGtgcggtaccctcacgacgtggtg<br />
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Clone into Nde1 + Xho1 1254 pTXB1-PT-ELK<br><br />
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[[Image:0726_Digestion.JPG]]<br />
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See lane 9 (insert and vector digested together - top is vector bottom is insert)<br />
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Cut out both bands and ligate together to transform <br />
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=== <u></u>Colony Screening<br> ===<br />
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Date:&nbsp;July 27, 2012<br />
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[[Image:0727_Digestion_2.JPG]]<br />
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Digested MiniPrep with RE Nde1 and Xho1<br />
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Expected size 1398 bp, see lanes 1-6<br />
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Samples 3, 5 and 6 may be right, submit sample 5 to sequencing<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Xiaojing.Wang