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1142-pLN6H-Tth-SSB - Revision history
2026-10-18T03:28:49Z
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Paul.xu at 17:00, 18 February 2014
2014-02-18T17:00:47Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1142-pLN6H-Tth-SSB''' ==<br />
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'''Purpose:''' Cloning of 1142-pLN6H-Tth-SSB <br />
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'''Date: '''2014-2-12---2014-2-14 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1142''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1142 2016 2033pcr 1-28-2014.JPG]]<br> <br />
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PCR using Tth Genomic DNA from ATCC (-80 oC freezer ATCC box)<br>TTh-SSB-Nde1-F<br>TTh-SSB-Xho1-R<br> <br />
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[[Image:1114 1142 1940 2016clone 2-12-2014.JPG]] <br />
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{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| Lane<br> <br />
| 3<br> <br />
| 4<br><br />
|-<br />
| <br> <br />
| PCR<br> <br />
| 2029 pLNC6H-sfGFP<br><br />
|-<br />
| <br> <br />
| colspan="2" | Nde1 + Xho1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2014-2-14 <br />
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Make miniprep and digest with&nbsp; Nde1 + Xho1 <br />
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[[Image:1114 1142digest 2-14-2014.JPG]]<br> <br />
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2014-2-18<br><br />
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digest with Sma1+Xho1<br><br />
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[[Image:1142digest_2-18-2014.JPG]]<br><br />
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c:20292029 pLNC6H-sfGFP<br><br />
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&lt;br&gt; <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> <br />
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[[Category:Cloning]]</div>
Paul.xu