https://eln.excellgen.com/index.php?action=history&feed=atom&title=1143-1151-1186-screening 1143-1151-1186-screening - Revision history 2026-10-18T02:20:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1143-1151-1186-screening&diff=7531&oldid=prev Paul.xu at 15:37, 14 December 2012 2012-12-14T15:37:49Z <p></p> <p><b>New page</b></p><div>Data:2012-11-15--2012-11-16 <br /> <br /> Exprimenter: Xiuli <br /> <br /> = =<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 402px; height: 77px;&quot;<br /> |-<br /> | 1143 <br /> | p8H.T7.Exo-1<br /> |-<br /> | 1151 <br /> | pET30b-sso7d-ToughTag<br /> |-<br /> | 1155 <br /> | pET28b-Ara-S1<br /> |-<br /> | 1156 <br /> | p8H-VentExo<br /> |-<br /> | 1186 <br /> | pDB.His.MBP-Xis<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> 4 ml sample each , 1143, 1151, 1155,1156,1186&amp;nbsp; induce with 0.5mM IPTG at&amp;nbsp; 37 oC for overnight. Supernants were loaded on the gel.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Supernant: <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;4&quot; | 1143&lt;br&gt; <br /> | colspan=&quot;4&quot; | 1151&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1186&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;4&quot; | p8H.T7.Exo-1&lt;br&gt; <br /> | colspan=&quot;4&quot; | pET30b-sso7d-ToughTag&lt;br&gt; <br /> | colspan=&quot;2&quot; | pDB.His.MBP-Xis&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | lane <br /> | 1 <br /> | 2 <br /> | 3 <br /> | 4 <br /> | 5 <br /> | 6 <br /> | 7 <br /> | 8 <br /> | 9 <br /> | 10 <br /> | 11<br /> |-<br /> | IPTG <br /> | 0 <br /> | 0 <br /> | 0.5 <br /> | 0.5 <br /> | 0 <br /> | 0 <br /> | 0.5 <br /> | 0.5 <br /> | 0 <br /> | 0.5 <br /> | 0<br /> |-<br /> | lane <br /> | C1 <br /> | C2 <br /> | E1 <br /> | E2 <br /> | C1 <br /> | C2 <br /> | E1 <br /> | E2 <br /> | C1 <br /> | E1 <br /> | MBP<br /> |}<br /> <br /> [[Image:1143-1186 front sup.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Supernant: <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;4&quot; | 1155&lt;br&gt; <br /> | colspan=&quot;4&quot; | 1156&lt;br&gt; <br /> | colspan=&quot;2&quot; | 1186&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | colspan=&quot;4&quot; | pET28b-Ara-S1&lt;br&gt; <br /> | colspan=&quot;4&quot; | p8H-VentExo&lt;br&gt; <br /> | colspan=&quot;2&quot; | pDB.His.MBP-Xis&lt;br&gt;<br /> |-<br /> | lane <br /> | 1 <br /> | 2 <br /> | 3 <br /> | 4 <br /> | 5 <br /> | 6 <br /> | 7 <br /> | 8 <br /> | 19 <br /> | 10 <br /> | 11<br /> |-<br /> | IPTG <br /> | 0 <br /> | 0 <br /> | 0 <br /> | 0.5 <br /> | 0.5 <br /> | 0 <br /> | 0 <br /> | 0.5 <br /> | 0.5 <br /> | 0 <br /> | 0.5<br /> |-<br /> | lane <br /> | MBP <br /> | C1 <br /> | C1 <br /> | E1 <br /> | E2 <br /> | C1 <br /> | C2 <br /> | E1 <br /> | E2 <br /> | C2 <br /> | E2<br /> |}<br /> <br /> &amp;nbsp;[[Image:1143-1186-back sup.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> 1156 and 1186 are expressed .&lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;421&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| width=&quot;413&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu