https://eln.excellgen.com/index.php?action=history&feed=atom&title=1163_pFUSE2-aKOD-CLIg-hk 2026-10-18T05:16:44Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1163_pFUSE2-aKOD-CLIg-hk&diff=5215&oldid=prev 2012-07-19T19:53:08Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1163 pFUSE2-aKOD-CLIg-hk&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1163 pFUSE2-aKOD-CLIg-hk <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-6-1 <br /> <br /> &#039;&#039;&#039;Notebook: &#039;&#039;&#039;0 <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1163 <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Jennifer Truong <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Date: 6/1/2012 <br /> <br /> [[Image:1163 1164 Amplifaction 06012012.jpg]] <br /> <br /> Synthesized template by gene synthesis, cut out band for cloning next week <br /> <br /> Date: July 19, 2012&lt;br&gt;<br /> <br /> Digested DNA (lane 1)&amp;nbsp;and pFUSE2-CLIg-hk,1162 lane 2)&amp;nbsp;with restriction enzymes AgeI + Nco1&lt;br&gt;<br /> <br /> Not a clear vector band, may need to redigest tomorrow. There is no fast digest AgeI, used NEB enzyme<br /> <br /> Cut out DNA band and saved in box<br /> <br /> [[Image:1163&amp;4_0719_Digestion.JPG]]<br /> <br /> === Colony Screening&lt;br&gt; ===<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div>

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