https://eln.excellgen.com/index.php?action=history&feed=atom&title=1164_pFUSE-aKOD-H-CHIg-hG1 2026-10-18T03:32:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1164_pFUSE-aKOD-H-CHIg-hG1&diff=5286&oldid=prev 2012-07-26T13:58:02Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1164 pFUSE-aKOD-H-CHIg-hG1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1164 pFUSE-aKOD-H-CHIg-hG1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;6/1/2012 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; 0 <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: &#039;&#039;&#039;1164 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Jennifer Truong&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> Date: 6/1/2012 <br /> <br /> [[Image:1163 1164 Amplifaction 06012012.jpg]] <br /> <br /> Synthesized template by gene synthesis, cut out band for cloning next week <br /> <br /> Date:&amp;nbsp;July 19, 2012&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> Digested DNA (lane 3)&amp;nbsp;and pFUSE-CHIg-hG1,1161 (lane 4)&amp;nbsp;with restriction enzymes EcoR1 + Nhe1<br /> <br /> Cut out bands in lanes 3 and 4. Saved in box and ligate together tomorrow<br /> <br /> [[Image:1163&amp;4_0719_Digestion.JPG]]&lt;br&gt;&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date:&amp;nbsp;July 25, 2012<br /> <br /> [[Image:1164_Screening.JPG]]<br /> <br /> Expected size ~500 bp, all samples are positive for insert<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Category:Cloning]]</div>

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