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1190 pCDNA3.1-Dest - Revision history
2026-10-18T02:22:56Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=1190_pCDNA3.1-Dest&diff=4546&oldid=prev
Paul.xu at 20:48, 9 June 2012
2012-06-09T20:48:53Z
<p></p>
<p><b>New page</b></p><div>5/20/2012<br> <br />
<br />
[[Image:1190 Kpn1 Xho1 digestion 5 20 2012.jpg]]<br> <br />
<br />
1: 374 pAd5-CMV-Dest-C6XHis<br> <br />
<br />
2: 524 pCDNA3.1-E1A<br> <br />
<br />
3: 335 pCDNA3.1<br> <br />
<br />
digest with Kpn1 + Xho1. <br />
<br />
digest 374 pAd5-CMV-Dest-C6XHis with Kpn1+ Xho1---&gt;gateway cassette<br>Digest pCDNA3.1, 335 with Kpn1 + Xho1 --&gt;vector<br>ligate and transform ccdb survival<br> <br />
<br />
<br> <br />
<br />
digest miniprep DNA with Kpn1+Xho1 <br />
<br />
[[Image:1190 digest detect 5-22-2012.JPG]] <br />
<br />
Clone #4 is good, digest with Nco1 + Not1, Not1 + BamH1, and BamH1. <br />
<br />
[[Image:1190 diges 5-22-2012.JPG]] <br />
<br />
{| cellspacing="1" cellpadding="1" border="1" style="width: 301px; height: 64px;"<br />
|-<br />
| <br><br />
| band 1 <br />
| band 2 <br />
| band 3<br />
|-<br />
| <br><br />
| Nco1+Not1 <br />
| Not1+BamH1 <br />
| BamH1<br />
|-<br />
| Expected Size <br />
| 621 <br />
| 773 <br />
| 703<br />
|}<br />
<br />
<br> Clone #4 has correct restriction sites. <br />
<br />
[[Image:1192 212 1186 1202 1190 5 27 2012.jpg]] <br />
<br />
5/28/2012 <br />
<br />
[[Image:1190 digestion 5 28 2012.jpg]] <br />
<br />
{| cellspacing="1" cellpadding="1" border="1" style="width: 235px; height: 80px;"<br />
|-<br />
| 1 <br />
| 2 <br />
| 3 <br />
| 4<br />
|-<br />
| Not1+ BamH1 <br />
| Nco1+Not1 <br />
| EcoR1+Pst1 <br />
| BamH1<br />
|-<br />
| <br><br />
| <br><br />
| <br><br />
| <br><br />
|}<br />
<br />
5/29&nbsp; 1190 are digested by Kpn1+Xho1 <br />
<br />
[[Image:1190 digest 5.29.2012.JPG]] <br />
<br />
<br> <br />
<br />
[[Image:P9His Not1 BamH1 5 30 2012.jpg]] <br />
<br />
pHis9 digest with Not1 + BamH1 <br />
<br />
<br><br />
<br />
6/9/2012<br><br />
<br />
Digest new 1190 pCDNA3.3-Des with Kpn1 + Xho1, or Not1 + BamH1<br />
<br />
[[Image:1190_Kpn1_Xho1_6_9_2012.jpg]]<br />
<br />
[[Image:1190_Not1_BamH1_6_9_2012.jpg]]<br />
<br />
[[Image:1190_Not1_BamH1_2_6_9_2012.jpg]]<br><br />
<br />
[[Category:Cloning]]</div>
Paul.xu