https://eln.excellgen.com/index.php?action=history&feed=atom&title=1192_pCDNA3.2 1192 pCDNA3.2 - Revision history 2026-10-18T05:36:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1192_pCDNA3.2&diff=4367&oldid=prev Paul.xu at 18:31, 31 May 2012 2012-05-31T18:31:01Z <p></p> <p><b>New page</b></p><div>5/25/2012 <br /> <br /> digest pCDNA3.1 with Nhe1 + Xba1&lt;br&gt;ligate to annealed pCDNA3.1-T + pCDNA3.1-B <br /> <br /> 5&#039; CTAGC GGATCC GAATTC ggtacc AAGCTT TTAATTAA Ctcgag GCGGCCGC GATATC T 3&#039;&lt;br&gt;3&#039; g CCTAGG CTTAAG ccatgg TTCGAA AATTAATT Gagctc CGCCGGCG CTATAG AGATCT 5&#039;&lt;br&gt; <br /> <br /> &lt;br&gt;pCDNA3.1-T&lt;br&gt;CTAGCGGATCCGAATTCggtaccAAGCTTTTAATTAACtcgagGCGGCCGCGATATCT&lt;br&gt;pCDNA3.1-B&lt;br&gt;TCTAGAGATATCGCGGCCGCctcgaGTTAATTAAAAGCTTggtaccGAATTCGGATCCg <br /> <br /> transform DH5a <br /> <br /> 5/27/2012 <br /> <br /> digest miniprep with PacI + Nde1 <br /> <br /> [[Image:212 1192 5 27 2012.jpg]] <br /> <br /> [[Image:1192 212 1186 1202 1190 5 27 2012.jpg]] <br /> <br /> 5/31/2012&lt;br&gt;[[Image:1192-2~4~6digest_5-31-2012.jpg]]&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu