https://eln.excellgen.com/index.php?action=history&feed=atom&title=1201_pCMV5-CymR 2026-10-18T07:46:28Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1201_pCMV5-CymR&diff=4528&oldid=prev 2012-06-08T17:34:14Z

<p></p> <p><b>New page</b></p><div>Cloning of pCMV5-CymR(1201)&lt;br&gt;Purpose: to clone pCMV5-CymR&lt;br&gt;Date: 2012-6-4 to 2012-6-&lt;br&gt;Notebook: Xiaojing Wang&lt;br&gt;Page: 004257&lt;br&gt;Clone ID: 1201&lt;br&gt;Experimenters: Xiaojing Wang&lt;br&gt;Results &lt;br&gt; <br /> <br /> digest 1202 pDNA3.1-CymR with Nde1 + BamH1--&amp;gt;Vector&lt;br&gt;digest 448 pLex-CMV5-CuO with Nde1 + BamH1--&amp;gt; insert (promoter)&lt;br&gt;[[Image:1201 i v digest 6.4.2012.JPG]]&lt;br&gt; <br /> <br /> Ligate and transform&lt;br&gt;Select 6 clones to mini-prep&lt;br&gt;1201 plasmids are digested by Nde1 + BamH1&lt;br&gt; <br /> <br /> [[Image:1201 digest 6.8.2012.JPG]]&lt;br&gt;<br /> <br /> 1201-1,-2 may be correct.&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang