https://eln.excellgen.com/index.php?action=history&feed=atom&title=1202_pDNA3.1-CymR 2026-10-18T02:22:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1202_pDNA3.1-CymR&diff=4369&oldid=prev 2012-05-31T20:18:25Z

<p></p> <p><b>New page</b></p><div>Cloning of pDNA3.1-CymR(1202)&lt;br&gt;Purpose: to clone pDNA3.1-CymR&lt;br&gt;Date: 2012-5-23 to 2012-5-&lt;br&gt;Notebook: Xiaojing Wang&lt;br&gt;Page: 004252&lt;br&gt;Clone ID: 1202&lt;br&gt;Experimenters: Xiaojing Wang&lt;br&gt;Results &lt;br&gt; <br /> <br /> digest pCDNA3.1, 335 with BamH1 + Xho1--&amp;gt; vector&lt;br&gt;digest 457 pET28b-CymR or pLenti-CymR 458 with BamH1 + Xho1--&amp;gt;insert&lt;br&gt; <br /> <br /> [[Image:1202 1203 i v 5.23.2012.JPG]] <br /> <br /> Ligate and transform&lt;br&gt;Select 6 clones to mini-prep&lt;br&gt;1202 plasmids are digested by BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1202 digest 5.29.2012.JPG]]&lt;br&gt; <br /> <br /> 1202-6,-9,10 may be correct. &lt;br&gt; <br /> <br /> &lt;br&gt;<br /> <br /> 1202-6 is digested by Nde1+BamH1 ---&amp;gt;band1&lt;br&gt;<br /> <br /> 1202-6 is digested by Nde1+Xho1 ---&amp;gt;band2&lt;br&gt;<br /> <br /> [[Image:1202 digest 5.31.2012.JPG]]&lt;br&gt;<br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang