https://eln.excellgen.com/index.php?action=history&feed=atom&title=1204_pET28b-pigIFN-alpha 2026-10-18T03:29:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1204_pET28b-pigIFN-alpha&diff=4513&oldid=prev 2012-06-07T18:24:20Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1204 pig IFN alpha&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of pig IFN alpha <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-5-29 <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1204&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date: 5-29-2012 <br /> <br /> PCR using Kapa HiFi. <br /> <br /> [[Image:1204 gene syn 5 29 2012.jpg]]&lt;br&gt; <br /> <br /> 6/2/2012 <br /> <br /> [[Image:1204 gene syn 6 2 2012.jpg]] <br /> <br /> cut out band and purify using gel extraction kit. digest with Nde1 + Xho1. ligate into Nde1 + Xho1 digested pET28b. <br /> <br /> transform DH5alpha <br /> <br /> 6/7/12<br /> <br /> digest miniprep with Nde1 + Xho1<br /> <br /> [[Image:1204_1234_6_7_2012.jpg]]<br /> <br /> === Colony Screening ===<br /> <br /> Date: &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Xiaojing.Wang