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1207-pDB.His.MBP-(3C) - Revision history
2026-10-18T02:19:26Z
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Jennifer.truong at 13:08, 21 August 2012
2012-08-21T13:08:12Z
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<p><b>New page</b></p><div>== '''Cloning of 1207-pDB.His.MBP-(3C)''' ==<br />
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'''Purpose:''' Cloning of 1207-pDB.His.MBP-(3C) <br />
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'''Date:&amp;nbsp;''' 2012-6-29---- <br />
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'''Notebook:''' <br />
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'''Page:''' <br />
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'''Clone ID: 1207''' <br />
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'''Experimenters:'''yingying lu <br />
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== '''Cloning''' ==<br />
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[[Image:1207 clone 6-29-2012.JPG]]<br> <br />
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lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,<br>ligate to annealled oligos<br>MBP-3C-T<br>T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g<br>MBP-3C-B<br>gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc<br><br />
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Date:&nbsp;August 17, 2012<br><br />
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lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,<br>ligate to annealled oligos<br>MBP-3C-T<br>T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g<br>MBP-3C-B<br>gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc<br />
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[[Image:1207_Vector_Digestion.JPG]]<br><br> <br />
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== '''Colony Screening''' ==<br />
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Date:&nbsp;August 20, 2012<br> <br />
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[[Image:1207 0820 Screening.JPG]] <br />
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Digest with Pst + Xba1---&gt;1.2 kb<br><br />
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Clones 1, 2, 3, 4, 5 and 6 may be correct<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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&lt;br&gt;<br> <br />
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[[Category:Cloning]]</div>
Jennifer.truong