https://eln.excellgen.com/index.php?action=history&feed=atom&title=1219_pCDNA3.3-Fc%283C%29 2026-10-18T02:19:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1219_pCDNA3.3-Fc(3C)&diff=5122&oldid=prev 2012-07-16T20:25:59Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Date: 2012-6-10&#039;&#039;&#039; <br /> <br /> &lt;br&gt; &#039;&#039;&#039;Notebook:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Page&#039;&#039;&#039;:&amp;nbsp; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; 1219 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; &lt;span style=&quot;font-weight: bold;&quot;&gt;Paul&lt;/span&gt;&lt;br&gt; <br /> <br /> == Cloning ==<br /> <br /> 6/10/2012.&amp;nbsp;&lt;br&gt; <br /> <br /> PCR: ssFc-BamH1-F1 + 10% ss-Fc-F5 + Fc-HRV3C-Nhe1-Kpn1-R6, template is pFUSE-hIgG1-Fc2-IAB, 1101&lt;br&gt; KAPA, 20 cycles.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1236 1219 6 10 2012.jpg]]&lt;br&gt; <br /> <br /> digest PCR product with BamH1+ Kpn1&lt;br&gt; <br /> <br /> [[Image:1236 1219 digestion 6 10 2012.jpg]] <br /> <br /> digest pCDNA3.3, 1208 with BamH1+ Kpn1, ligate and transform Top10&lt;br&gt; <br /> <br /> == Colony Screening by Restriction Digestion&lt;br&gt; ==<br /> <br /> [[Image:1219 Digestion.JPG]] <br /> <br /> Digested lanes 1-6 with BAMHI and EcoRI and digested lanes 7-12 with Nhe1, positive for lanes samples 2, 4, 5 and 6. <br /> <br /> Submitted sample 4 for sequencing. <br /> <br /> <br /> <br /> 2012-7-16<br /> <br /> 1219 digest detect <br /> <br /> [[Image:1219_digest_detect_7-16-2012.JPG]]<br /> <br /> [[Category:Cloning]]</div>

Paul.xu