https://eln.excellgen.com/index.php?action=history&feed=atom&title=1220_pFUSE2-CLIg-hk-zeo 1220 pFUSE2-CLIg-hk-zeo - Revision history 2026-10-18T05:19:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1220_pFUSE2-CLIg-hk-zeo&diff=4387&oldid=prev Jennifer.truong: Created page with "== '''Cloning of 1220 pFUSE2-CLIg-hk-zeo''' == '''Purpose:''' make pFUSE2-CLIg-hk-zeo vector<br> '''Date: '''2012-6-3 '''Notebook:&nbsp;''' '''Page:''' '''Clone ID:1220..." 2012-06-03T19:28:55Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1220 pFUSE2-CLIg-hk-zeo&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; make pFUSE2-CLIg-hk-zeo vector&lt;br&gt; &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-6-3 &#039;&#039;&#039;Notebook: &#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1220...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1220 pFUSE2-CLIg-hk-zeo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; make pFUSE2-CLIg-hk-zeo vector&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-6-3 <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1220&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> <br /> <br /> 6/3/2012&lt;br&gt; <br /> <br /> &lt;br&gt;digest 1161 pFUSE-CHIg-hG1 (Lane 4, 5, 6) with Hpa1 + Nde1 ---&amp;gt; zeocin insert&lt;br&gt;the size of zeocin insert should be ~1.6 kb. this one looks like 5.5 kb, maybe not digested?<br /> <br /> [[Image:1208 6 3 12012.jpg]] <br /> <br /> [[Image:1208 6 3 12012 1.jpg]] <br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> digest pFUSE2-CLIg-hk 1162 with Hpa1 + Nde1 ---&amp;gt; vector&lt;br&gt;<br /> <br /> ligate, transform DH5alpha&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Jennifer.truong