Paul.xu at 18:53, 17 July 2012

2012-07-17T18:53:26Z

New page

== '''Cloning of 1222-pCMV5-Fc(3C)-Dest''' ==

'''Purpose:''' Cloning of 1222-pCMV5-Fc(3C)-Dest

'''Date: '''2012-7-10---2012-7-17

'''Notebook:0'''

'''Page:'''

'''Clone ID:1222'''

'''Experimenters:''' yingying

== '''Results''' ==

=== subcloning ===

2012-7-9 

[[Image:1222 clone 7-9-2012.JPG]] 

{| cellspacing="1" cellpadding="1" border="1" style="width: 406px; height: 96px;"
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| 
| lane 1 
| lane 2 
|-
| 
| 448 pLex-CMV5-CuO 
| 1223 pCDNA3.3-Fc(3C)-Dest 
|-
| enzymes 
| colspan="2" |       Nde1 + BamH1 
|}

 

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=== Colony Screening ===

Date: 2012-7-12

Make miniprep and digest with Nde1 + BamH1

[[Image:1151+1222 digest detect 7-12-2012.JPG]]

clone 3,4,5,6,7,8 may be right

 

2012-7-17

clone 3 digest detect:

[[Image:1222 digest detec 7-17-2012.JPG]]

lane 1:Nde1+BamH1

lane 2:Nde1+Hind111

clone 3 right 

== '''Result intepretation/Summary''' ==

*&amp;lt;br&amp;gt;

== '''Conclusion''' ==

&amp;lt;br&amp;gt;

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[[Category:Cloning]]

1222-pCMV5-Fc(3C)-Dest - Revision history

Paul.xu at 18:53, 17 July 2012