https://eln.excellgen.com/index.php?action=history&feed=atom&title=1225_pET28b-T7-Endo1 2026-10-18T02:20:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1225_pET28b-T7-Endo1&diff=4563&oldid=prev 2012-06-11T18:15:42Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of T7 endonuclease 1, gene 3 into pET28b&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of T7 endonuclease 1, gene 3 into pET28b<br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; 06/08/2012&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1225&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; Jennifer Truong&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning&#039;&#039;&#039; ==<br /> <br /> 06/08/2012: Amplified gene with PCR&amp;nbsp;(KAPA&amp;nbsp;enzyme, 20 cycles) Cut out circled bands for purification and digestion <br /> <br /> [[Image:1225 amp.JPG]] <br /> <br /> Digested purified DNA and pET28b vector with Nde1 and Xho1, cut out bands for ligation (lanes 3 and 4)&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Colony Screening&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> [[Image:1143_1225_Digestion.JPG]]&lt;br&gt; <br /> <br /> DNA very weak, submitted sample #1 (lane 7)&amp;nbsp;for sequencing<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu